Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. Signal., 22 December 2009
[DOI: 10.1126/scisignal.2000389]

Supplementary Materials for:

Tumor Suppression by PTEN Requires the Activation of the PKR-eIF2α Phosphorylation Pathway

Zineb Mounir, Jothi Latha Krishnamoorthy, Gavin P. Robertson, Donalyn Scheuner, Randal J. Kaufman, Maria-Magdalena Georgescu, Antonis E. Koromilas*

*To whom correspondence should be addressed. E-mail: antonis.koromilas{at}mcgill.ca

This PDF file includes:

  • Fig. S1. Effect of doxycycline on the phosphorylation of eIF2α in parental U87 and U251 cells.
  • Fig. S2. Phosphatase-deficient mutants of PTEN do not affect the phosphorylation of S6 or 4EBP-1.
  • Fig. S3. The kinases PERK and GCN2 are not activated by PTEN.
  • Fig. S4. Detection of the activation of PKR by PTEN in vivo.
  • Fig. S5. Role of PKR in PTEN-mediated inhibition of PC-3 cell proliferation.
  • Fig. S6. Role of phosphorylated eIF2α in PTEN-mediated inhibition of PC-3 cell proliferation.
  • Fig. S7. Lack of a physical interaction between PKR and PTEN.
  • Fig. S8. The PKR-eIF2α phosphorylation pathway acts downstream of PTEN.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.04 MB

Citation: Z. Mounir, J. L. Krishnamoorthy, G. P. Robertson, D. Scheuner, R. J. Kaufman, M.-M. Georgescu, A. E. Koromilas, Tumor Suppression by PTEN Requires the Activation of the PKR-eIF2α Phosphorylation Pathway. Sci. Signal. 2, ra85 (2009).

© 2009 American Association for the Advancement of Science

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882