Direct Response to Notch Activation: Signaling Crosstalk and Incoherent Logic

Alena Krejčí* Fred Bernard,* Ben E. Housden,* Stephanie Collins, Sarah J. Bray†

This PDF file includes:

• Methods
• References
• Fig. S1. Array data plots and validation assays.
• Fig. S2. ChIP peaks are enriched for high affinity and multiple Su(H) sites.
• Fig. S3. Enrichment of GO categories amongst Ras responsive genes in Drosophila hemocytes and Notch responsive genes in Kc cells.
• Fig. S4. Notch targets include apoptosis inducing genes.
• Fig. S5. dm/myc is a Notch target and required for Notch induced proliferation.
• Fig. S6. Enhancers from aos and Egfr are regulated by Notch in vivo.
• Table S1. Regulated Peak genes (RPG): Genes regulated by Notch in DmD8 cells (EDTA stimulated and/or DFK inhibited) that are associated with peaks of Su(H).
• Table S2. Assigned peaks (AP): Genes associated with Su(H) ChIP that are regulated by Notch in DmD8 and/or Kc cells.
• Table S3. Regulated genes (RG): Genes stimulated by Notch activation and/or inhibited with DFK with no associated peak of Su(H) binding in DmD8 cells.

Technical Details

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Supplementary Materials (Excel File)

• Table S1. Regulated Peak genes (RPG): Genes regulated by Notch in DmD8 cells (EDTA stimulated and/or DFK inhibited) that are associated with peaks of Su(H)(Excel file).
• Table S2. Assigned peaks (AP): Genes associated with Su(H) ChIP that are regulated by Notch in DmD8 and/or KC cells (Excel file).
• Table S3. Regulated genes (RG): Genes stimulated by Notch activation and/or inhibited with DFK with no associated peak of Su(H) binding in DmD8 cells (Excel file).

Format: Microsoft Excel

Size: 283 KB (compressed); 1,864 KB (decompressed)

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*These authors contributed equally to this work.
†To whom correspondence should be addressed. E-mail, sjb32{at}cam.ac.uk

Citation: A. Krejčí, F. Bernard, B. Housden, S. Collins, S. J. Bray, Direct Response to Notch Activation: Signaling Crosstalk and Incoherent Logic. Sci. Signal. 2, ra1 (2009).

© 2009 American Association for the Advancement of Science