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Sci. Signal., 27 January 2009
[DOI: 10.1126/scisignal.2000140]

Direct Response to Notch Activation: Signaling Crosstalk and Incoherent Logic

Alena Krejčí* Fred Bernard,* Ben E. Housden,* Stephanie Collins, Sarah J. Bray†

This PDF file includes:

  • Methods
  • References
  • Fig. S1. Array data plots and validation assays.
  • Fig. S2. ChIP peaks are enriched for high affinity and multiple Su(H) sites.
  • Fig. S3. Enrichment of GO categories amongst Ras responsive genes in Drosophila hemocytes and Notch responsive genes in Kc cells.
  • Fig. S4. Notch targets include apoptosis inducing genes.
  • Fig. S5. dm/myc is a Notch target and required for Notch induced proliferation.
  • Fig. S6. Enhancers from aos and Egfr are regulated by Notch in vivo.
  • Table S1. Regulated Peak genes (RPG): Genes regulated by Notch in DmD8 cells (EDTA stimulated and/or DFK inhibited) that are associated with peaks of Su(H).
  • Table S2. Assigned peaks (AP): Genes associated with Su(H) ChIP that are regulated by Notch in DmD8 and/or Kc cells.
  • Table S3. Regulated genes (RG): Genes stimulated by Notch activation and/or inhibited with DFK with no associated peak of Su(H) binding in DmD8 cells.

Technical Details

Format: Adobe Acrobat PDF

Size: 2,078 KB

[Download PDF]

Supplementary Materials (Excel File)

  • Table S1. Regulated Peak genes (RPG): Genes regulated by Notch in DmD8 cells (EDTA stimulated and/or DFK inhibited) that are associated with peaks of Su(H)(Excel file).
  • Table S2. Assigned peaks (AP): Genes associated with Su(H) ChIP that are regulated by Notch in DmD8 and/or KC cells (Excel file).
  • Table S3. Regulated genes (RG): Genes stimulated by Notch activation and/or inhibited with DFK with no associated peak of Su(H) binding in DmD8 cells (Excel file).
  • Format: Microsoft Excel

    Size: 283 KB (compressed); 1,864 KB (decompressed)

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    *These authors contributed equally to this work.
    †To whom correspondence should be addressed. E-mail, sjb32{at}cam.ac.uk

    Citation: A. Krejčí, F. Bernard, B. Housden, S. Collins, S. J. Bray, Direct Response to Notch Activation: Signaling Crosstalk and Incoherent Logic. Sci. Signal. 2, ra1 (2009).

    © 2009 American Association for the Advancement of Science


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