Dok-7 Activates the Muscle Receptor Kinase MuSK and Shapes Synapse Formation

Akane Inoue, Kiyoko Setoguchi, Yosuke Matsubara, Kumiko Okada, Nozomi Sato, Yoichiro Iwakura, Osamu Higuchi, Yuji Yamanashi*

*To whom correspondence should be addressed. E-mail: yyamanas{at}ims.u-tokyo.ac.jp

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• Fig. S1. The pHSA-Dok-7-EGFP transgene is overexpressed in skeletal muscle.
• Fig. S2. The number of myofibers was comparable in WT and Dok-7 Tg mice.
• Fig. S3. AChR clusters formed only in Dok-7^–/– myotubes that express Dok-7-EGFP.
• Fig. S4. Transcripts for endogenous Dok-7 were distributed uniformly throughout the muscle at E18.5.
• Fig. S5. The Myc epitope appropriately increased the molecular mass of phosphorylated MuSK-cyt.
• Fig. S6. Dok-7 activated MuSK-cyt.
• Fig. S7. MuSK was not activated by a Dok-7 deletion mutant lacking the PH domain or the Dok-family protein Dok-1.
• Fig. S8. The pHSA-Dok-7-Myc transgene is overexpressed in skeletal muscle.

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Citation: A. Inoue, K. Setoguchi, Y. Matsubara, K. Okada, N. Sato, Y. Iwakura, O. Higuchi, Y. Yamanashi, Dok-7 activates the muscle receptor kinase MuSK and shapes synapse formation. Sci. Signal. 2, ra7 (2009).

© 2009 American Association for the Advancement of Science