Measurement of Phosphorylated Extracellular Signal–Regulated Kinase 1 and 2 in an Undergraduate Teaching Laboratory with ALPHAscreen Technology (Presentation)

Debbie L. Hay^*

School of Biological Sciences, University of Auckland, Auckland, New Zealand.

*Corresponding author. Telephone, +64-9-373-7599; fax, +64-9-373-7414; e-mail, dl.hay{at}auckland.ac.nz

Presentation. Signal Transduction in Drug Discovery.

This presentation was used in conjunction with the laboratory exercise for a class of 300 second-year biochemistry students and outlines the use of technologies such as ALPHAscreen in drug discovery, their advantages over conventional techniques, and considerations for high-throughput assays, as well as providing some practical tips.

References

J. M. Berg, J. L. Tymoczko, L. Stryer, Biochemistry (Freeman, New York, ed. 6, 2007).

H. Lodish, A. Berk, C. A. Kaiser, M. Krieger, M. P. Scott, A. Bretscher, H. Ploegh, P. Matsudaira, Molecular Cell Biology (Freeman, New York, ed. 6, 2008).

J. G. Lombardino, J. A. Lowe III, The role of the medicinal chemist in drug discovery—then and now. Nat. Rev. Drug Discov. 3, 853–862 (2004).

J. P. Overington, B. Al-Lazikani, A. L. Hopkins, How many drug targets are there? Nat. Rev. Drug Discov. 5, 993–996 (2006).

S. Parra, R. A. Bond, Inverse agonism: From curiosity to accepted dogma, but is it clinically relevant? Curr. Opin. Pharmacol. 7, 146–150 (2007).

S. Siehler, Cell-based assays in GPCR drug discovery. Biotechnol. J. 3, 471–483 (2008).

C. Williams, cAMP detection methods in HTS: Selecting the best from the rest. Nat. Rev. Drug Discov. 3, 125–135 (2004).

[Download Presentation in PDF format]

Technical Details

Format: PDF

Size: 1.9 MB

Requirements: Adobe Acrobat Reader

Educational Details

Learning Resource Type: Digital Presentation

Context: Undergraduate lower division, undergraduate upper division

Intended Users: Teacher

Intended Educational Use: Teach, plan, assess

Discipline: Pharmacology, cell biology, biochemistry

Keywords: Signal transduction, cell culture, G protein–coupled receptor, extracellular signal regulated kinase, drug discovery

Citation: D. L. Hay, Measurement of Phosphorylated Extracellular Signal–Regulated Kinase 1 and 2 in an Undergraduate Teaching Laboratory with ALPHAscreen Technology. Sci. Signal. 2, tr1 (2009).

© 2009 American Association for the Advancement of Science