Complexity in Transcription Control at the Activation Domain–Mediator Interface

Michael A. Balamotis, Mario A. Pennella, Jennitte L. Stevens, Bohdan Wasylyk, Andrew S. Belmont, and Arnold J. Berk*

*To whom correspondence should be addressed. E-mail: berk{at}mbi.ucla.edu

This PDF file includes:

• Methods and Materials
• Fig. S1. Phenotype of E9.5 Med23^–/– mouse embryos.
• Fig. S2. Overlap of genes expressed in ES and MEF cells affected by the Med23 KO.
• Fig. S3. Egr1 mRNAs and Egr1 nascent pre-mRNAs (intron containing) per cell.
• Fig. S4. ChIP of Pol II and mediator at the EF2 promoter in WT and KO ES and MEF cells after addition of serum to serum-starved cells.
• Fig. S5. TCF ChIP in WT ES and MEF cells.
• Fig. S6. Effect of overexpression of full-length TCFs in ES and MEF KO cells.
• Fig. S7. Gal4-TCF AD protein levels and activation function in ES KO, H1299, and HeLa cells.
• Fig. S8. Mediator colocalization with serine mutants of ELK1, SAP1, and NET with MAPK activation.
• Table S1. Activator-mediator subunit interactions.
• Table S2. Lethal phenotype of the homozygous Med23 knockout.
• Table S3. Primers for mRNA and ChIP qRT-PCR analyses.
• References
• Movie S1. Kinetics of mediator binding to ELK1 AD in vivo after serum addition.

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Citation: M. A. Balamotis, M. A. Pennella, J. L. Stevens, B. Wasylyk, A. S. Belmont, A. J. Berk, Complexity in Transcription Control at the Activation Domain–Mediator Interface. Sci. Signal. 2, ra20 (2009).

© 2009 American Association for the Advancement of Science