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Sci. Signal., 9 June 2009
[DOI: 10.1126/scisignal.2000259]

Supplementary Materials for:

PI3Kγ Adaptor Subunits Define Coupling to Degranulation and Cell Motility by Distinct PtdIns(3,4,5)P3 Pools in Mast Cells

Thomas Bohnacker, Romina Marone, Emilie Collmann, Ronan Calvez, Emilio Hirsch, Matthias P. Wymann*

*To whom correspondence should be addressed. E-mail: Matthias.Wymann{at}UniBas.ch

This PDF file includes:

  • Supplementary Text
  • Materials and Methods
  • Fig. S1. Tissue distribution of p110γ and its adaptor subunits (additional tissues).
  • Fig. S2. p84 is stabilized by p110γ in mast cells.
  • Fig. S3. Restoration of p110γ does not affect the abundance of endogenous p84 protein.
  • Fig. S4. Adenosine receptor ligands and PI3Kγ signaling leads to phosphorylation of Akt.
  • Fig. S5. Illustration of the gating procedures used in single-cell degranulation assays.
  • Fig. S6. p101:p110γ–dependent endocytosis of PtdIns(3,4,5)P3 is associated with microtubules.
  • Fig. S7. Quantification of PHBtk-GFP probes at the plasma membrane after low-level stimulation of BMMCs with IgE and antigen.
  • Fig. S8. Activation of Akt and localization of PtdIns(3,4,5)P3 in J774 cells.
  • Table S1. Sequences of primers used for cloning, antigen production, qPCR, and RT-PCR.
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1,777 KB

Other Supplementary Materials for this manuscript include the following:

Movies m1 through m9 and movie descriptions

  • Descriptions of movies m1 to m9. (Adobe Acrobat PDF; file size 151 KB)
  • Movie m1. (filename: m1.p84_z_stack.mov; file size 147 KB; format: QuickTime Movie)
  • Movie m2. (filename: m2.p101_z_stack.mov; file size 147 KB; QuickTime Movie)
  • Movie m3. (filename: m3.p84_ade.mov; file size 5.19 MB; QuickTime Movie)
  • Movie m4. (filename: m4.p101_ade.mov; file size 5.39 MB; QuickTime Movie)
  • Movie m5. (filename: m5.p84_DNP_ade.mov; file size 1.79 MB; QuickTime Movie)
  • Movie m6. (filename: m6.p101_DNP_ade.mov; file size 2.19 MB; QuickTime Movie)
  • Movie m7. (filename: m7.p101_ade_SIP.mov; file size 4.99 MB; QuickTime Movie)
  • Movie m8. (filename: m8.p101_ade_NOC.mov; file size 5.14 MB; QuickTime Movie)
  • Movie m9. (filename: m9.p101_ade_NOC_SIP.mov; file size 3.92 MB; QuickTime Movie)

Citation: T. Bohnacker, R. Marone, E. Collmann, R. Calvez, E. Hirsch, M. P. Wymann, PI3Kγ adaptor subunits define coupling to degranulation and cell motility by distinct PtdIns(3,4,5)P3 pools in mast cells. Sci. Signal. 2, ra27 (2009).

© 2009 American Association for the Advancement of Science


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