PKC-θ Modulates the Strength of T Cell Responses by Targeting Cbl-b for Ubiquitination and Degradation

Thomas Gruber, Natascha Hermann-Kleiter, Reinhard Hinterleitner, Friedrich Fresser, Rainer Schneider, Günther Gastl, Josef M. Penninger, Gottfried Baier*

*To whom correspondence should be addressed. E-mail: gottfried.baier{at}i-med.ac.at

This PDF file includes:

• Fig. S1. PKCθ-deficient T cells exhibit hyporesponsiveness.
• Fig. S2. The PKC-θ–dependent recall response is restored to WT levels in DKO T cells.
• Fig. S3. PKC-θ phosphorylates the TKB domain of Cbl-b in vitro.
• Table S1. Effects of single or double deficiencies in cblb and PKCθ on cellularity.
• Table S2. Relative abundance of CD25, CD44, and CD69 on CD3⁺ T cells by genotype.
• Table S3. Specific interactions between PKC-θ and Cbl-b in the GAL4 yeast two-hybrid system.
• References

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Citation: T. Gruber, N. Hermann-Kleiter, R. Hinterleitner, F. Fresser, R. Schneider, G. Gastl, J. M. Penninger, G. Baier, PKC-θ modulates the strength of T cell responses by targeting Cbl-b for ubiquitination and degradation. Sci. Signal. 2, ra30 (2009).

© 2009 American Association for the Advancement of Science