Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 30 June 2009
[DOI: 10.1126/scisignal.2000135]

Supplementary Materials for:

PKCε Regulation of an α5 Integrin–ZO-1 Complex Controls Lamellae Formation in Migrating Cancer Cells

Saara Tuomi, Anja Mai, Jonna Nevo, Jukka O. Laine, Vesa Vilkki, Tiina J. Öhman, Carl G. Gahmberg, Peter J. Parker, Johanna Ivaska*

*To whom correspondence should be addressed. E-mail: Johanna.ivaska{at}vtt.fi

This PDF file includes:

  • Materials and Methods
  • Fig. S1. α5 integrin is increased on the cell surface and contributes to migration of NCI-H460 cells.
  • Fig. S2. α5 integrin, but not α2 integrin, is recruited to the lamellae with ZO-1 upon wounding.
  • Fig. S3. Verification of siRNA efficacy.
  • Fig. S4. α5 integrin and ZO-1 colocalize in the lamellae of breast cancer cells; silencing of ZO-1 influences focal adhesion morphology.
  • Fig. S5. ZO-1 knock-down increases Rac activity.
  • Fig. S6. α5WT- and α5PPAA-expressing cells adhere equally well to fibronectin but differ in lamellae formation.
  • Fig. S7. α5 integrin and ZO-1 form a complex in a fraction of lung cancers.
  • Fig. S8. ZO-1 phosphorylation is increased in subconfluent cells; PKC inhibition impairs recruitment of α5 integrin and ZO-1 to lamellae; α5 mediates adhesion to fibronectin.
  • Fig. S9. Loss of PKCε does not influence internalization of α5 integrin from the cell surface.
  • Fig. S10. PKCε regulates the subcellular localization of ZO-1 through the putative PKC site Ser168.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 16 MB

Other Supplementary Materials for this manuscript include the following:

Movies S1 through S5 and movie descriptions

  • Descriptions of movies S1 to S5. (Adobe Acrobat PDF; file size 119 KB)
  • Movie S1. (filename: Movie_S1.mov; file size 896 KB; QuickTime Movie)
  • Movie S2. (filename: Movie_S2.mov; file size 5.98 MB; QuickTime Movie)
  • Movie S3. (filename: Movie_S3.mov; file size 5.67 MB; QuickTime Movie)
  • Movie S4. (filename: Movie_S4.mov; file size 876 KB; QuickTime Movie)
  • Movie S5. (filename: Movie_S5.mov; file size 1.11 MB; QuickTime Movie)

Citation: S. Tuomi, A. Mai, J. Nevo, J. O. Laine, V. Vilkki, T. J. Öhman, C. G. Gahmberg, P. J. Parker, J. Ivaska, PKCε regulation of an α5 integrin–ZO-1 complex controls lamellae formation in migrating cancer cells. Sci. Signal. 2, ra32 (2009).

© 2009 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882