Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. Signal., 18 August 2009
[DOI: 10.1126/scisignal.2000448]

Supplementary Materials for:

The Plant NADPH Oxidase RBOHD Mediates Rapid Systemic Signaling in Response to Diverse Stimuli

Gad Miller, Karen Schlauch, Rachel Tam, Diego Cortes, Miguel A. Torres, Vladimir Shulaev, Jeffery L. Dangl, Ron Mittler*

*To whom correspondence should be addressed. E-mail: ronm{at}unr.edu

This PDF file includes:

  • Fig. S1. A model of the ROS-mediated autopropagating long-distance rapid signal in plants.
  • Fig. S2. qRT-PCR analysis of the rapid systemic expression of Zat12, ankyrin repeat family protein, WRKY18, WRKY40, and WRKY53 in wild type and mutants deficient in RBOHD (rbohD), RBOHF (rbohF), or RBOHC (rbohC).
  • Fig. S3. Detection of the rapid systemic signal in response to local application of high light intensity, cold, heat, or salinity stresses.
  • Table S1. Pharmacological treatments used to study the rapid systemic signal with the experimental design shown in Fig. 3.
  • Table S2. Transcripts down-regulated in systemic leaves of Arabidopsis plants 10 min after wounding.
  • Descriptions of Movies S1 to S5.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 819 KB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1. Suppression of the rapid wound-induced systemic signal in Zat12::Luc and Zat12::Luc/rbohD rosette leaves.
  • Movie S2. Rapid wound-induced systemic signal in inflorescences of control Zat12::Luc and Zat12::Luc/rbohD plants.
  • Movie S3. Local wound-induced accumulation of ROS in wild-type and rbohD plants.
  • Movie S4. Delay in the progression of the rapid systemic signal by application of DPI at distal location.
  • Movie S5. Delay in the progression of the rapid systemic signal by application of catalase at distal location.

[Download Movies (Compressed)]

Technical Details

Format: Movie (mov) (Zipped)

Size: 1.55 MB

Citation: G. Miller, K. Schlauch, R. Tam, D. Cortes, M. A. Torres, V. Shulaev, J. L. Dangl, R. Mittler, The plant NADPH oxidase RBOHD mediates rapid systemic signaling in response to diverse stimuli. Sci. Signal. 2, ra45 (2009).

© 2009 American Association for the Advancement of Science

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882