Proteomic Analysis of Integrin-Associated Complexes Identifies RCC2 as a Dual Regulator of Rac1 and Arf6

Jonathan D. Humphries, Adam Byron, Mark D. Bass, Sue E. Craig, John W. Pinney, David Knight, Martin J. Humphries*

*To whom correspondence should be addressed. E-mail: martin.humphries{at}manchester.ac.uk

This PDF file includes:

• Methods
• Fig. S1. Disruption of the actin cytoskeleton does not affect recruitment of talin and paxillin to isolated α₅β₁–FN complexes.
• Fig. S2. Knockdown of RCC2 accelerates adhesion formation.
• Fig. S3. Knockdown of RCC2 augments FN-mediated cell spreading.
• Table S1. Hierarchical clustering analysis of proteins identified in FN, VCAM-1, and VCAM-1(D40A) affinity purifications (full data sets).
• Table S2. Specifically enriched proteins identified in FN and VCAM-1 affinity purifications.
• Table S3. Protein-protein interaction network statistics.
• References

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Other Supplementary Materials for this manuscript include the following:

• File 1. Table S1 (Microsoft Excel format)
• File 2. Table S2 (Microsoft Excel format)
• File 3. Interaction network model for the α₅β₁–FN complex (XGMML format).
• File 4. Interaction network model for the α₄β₁–VCAM-1 complex (XGMML format).

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Technical Details

Format: Microsoft Excel and XGMML formats [compressed]

The XGMML format is used to display graphs and networks and can be viewed by the Cytoscape program, which can be freely downloaded from http://www.cytoscape.org/.

Size: 246 KB

Citation: J. D. Humphries, A. Byron, M. D. Bass, S. E. Craig, J. W. Pinney, D. Knight, M. J. Humphries, Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Sci. Signal. 2, ra51 (2009).

© 2009 American Association for the Advancement of Science