Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. Signal., 15 September 2009
[DOI: 10.1126/scisignal.2000370]

Supplementary Materials for:

Novel p47phox-Related Organizers Regulate Localized NADPH Oxidase 1 (Nox1) Activity

Davide Gianni, Begoña Diaz, Nicolas Taulet, Bruce Fowler, Sara A. Courtneidge,* Gary M. Bokoch*

*To whom correspondence should be addressed. E-mail: bokoch{at} (G.M.B.) and courtneidge{at} (S.A.C.)

This PDF file includes:

  • Fig. S1. Myc-tagged transfected organizer subunits are expressed in comparable amounts in reconstituted HEK293 cells.
  • Fig. S2. DLD1 cells only express the Nox1 member of the NADPH oxidase family, the NoxA1 activator, and Tks4 (and small amounts of Tks5) as members of the p47phox organizer superfamily.
  • Fig. S3. The presence of Tks5 single SH3 domain inactivating mutations (M1 to M5), or the TksM1M2 double mutation did not affect the ability of Tks5 to support Nox1-dependent ROS generation and to bind NoxA1.
  • Fig. S4. Tks4-specific siRNA efficiently knocks down Tks4 protein at 72 and 96 hours and significantly reduces the ability of DLD1 cells to degrade ECM.
  • Fig. S5. Nox1-specific siRNAs efficiently block ROS generation, invadopodia formation, and ECM degradation in DLD1 cells and this effect can be rescued by overexpression of murine Nox1 (mNox1).
  • Fig. S6. In DLD1 cells, Nox1 and phalloidin- and Nox1 and cortactin-positive structures are ventral organelles that have the characteristics of invadopodia.
  • Fig. S7. NoxO1-GFP does not localize to invadopodia in DLD1 cells and invadopodia formation in NoxO1-transfected cells is greatly reduced.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 711 KB

Citation: D. Gianni, B. Diaz, N. Taulet, B. Fowler, S. A. Courtneidge, G. M. Bokoch, Novel p47phox-related organizers regulate localized NADPH oxidase 1 (Nox1) activity. Sci. Signal. 2, ra54 (2009).

© 2009 American Association for the Advancement of Science

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882