Leishmania GP63 Alters Host Signaling Through Cleavage-Activated Protein Tyrosine Phosphatases

Maria Adelaida Gomez, Irazu Contreras, Maxime Hallé, Michel L. Tremblay, Robert W.
McMaster, Martin Olivier*

*To whom correspondence should be addressed. E-mail: martin.olivier{at}mcgill.ca

This PDF file includes:

• Methods
• Fig. S1. Distribution of PTPs after Leishmania infection.
• Fig. S2. Pattern of PTP activity upon infection with different Leishmania species.
• Fig. S3. Putative cleavage sites in the PTPs.
• Fig. S4. Targeted deletion of putative cleavage sites.
• Fig. S5. Recombinant GP63 mediates PTP cleavage in cell lysates.
• Fig. S6. MβCD cytotoxicity.
• Fig. S7. Colocalization of GP63 and the lipid raft marker CTxB.
• Fig. S8. Leishmania infection triggers PTP1B cleavage in SHP-1^–/– MØs.
• Fig. S9. Cleaved PTPs partially disappear after 24 hours of infection.
• Table S1. Putative GP63 cleavage sites.
• References

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Technical Details

Format: Adobe Acrobat PDF

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Citation: M. A. Gomez, I. Contreras, M. Hallé, M. L. Tremblay, R. W. McMaster, M. Olivier, Leishmania GP63 Alters Host Signaling Through Cleavage-Activated Protein Tyrosine Phosphatases. Sci. Signal. 2, ra58 (2009).

© 2009 American Association for the Advancement of Science