Coordinated Responses to Oxygen and Sugar Deficiency Allow Rice Seedlings to Tolerate Flooding

Kuo-Wei Lee, Peng-Wen Chen, Chung-An Lu, Shu Chen, Tuan-Hua David Ho, Su-May Yu*

*To whom correspondence should be addressed. E-mail: sumay{at}imb.sinica.edu.tw

This PDF file includes:

• Fig. S1. Dendrogram showing the evolutionary relationship of members of the rice CIPK family and other Snf1-related protein kinases from rice, mammals and yeast.
• Fig. S2. Genotyping identified two allelic Tos17-tagged CIPK homozygous (–/–) and heterozygous (+/–) Nipponbare rice mutants.
• Fig. S3. Amino acid sequence of CIPK15 closely resembles that of the rice SnRK1A, mammalian AMPKα1 and yeast Snf1 protein kinases.
• Fig. S4. Amino acid sequence of CIPK15 resembles that of protein kinases upstream of SNF1 and AMPK.
• Fig. S5. Sucrose rescues underwater seedling shoot development in the cipk15 mutant.
• Fig. S6. CIPK15 controls underwater root development.
• Fig. S7. CIPK15 control mature plant growth under partially-flooded conditions.
• Fig. S8. Seedling hypoxia tolerance varies among rice varieties.
• Fig. S9. ATP is consumed more slowly in WT and cipk15 embryos germinated under water than in air.
• Table S1. The IRRI Germplasm Center (IRGC) accession number of rice germplasms used in the present study.
• Table S2. Primers used in the present study.

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Citation: K.-W. Lee, P.-W. Chen, C.-A. Lu, S. Chen, T.-H. D. Ho, S.-M. Yu, Coordinated responses to oxygen and sugar deficiency allow rice seedlings to tolerate flooding. Sci. Signal. 2, ra61 (2009).

© 2009 American Association for the Advancement of Science