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Sci. Signal., 27 October 2009
[DOI: 10.1126/scisignal.2000442]

Supplementary Materials for:

Increased MKK4 Abundance with Replicative Senescence Is Linked to the Joint Reduction of Multiple MicroRNAs

Bernard S. Marasa, Subramanya Srikantan, Kiyoshi Masuda, Kotb Abdelmohsen, Yuki Kuwano, Xiaoling Yang, Jennifer L. Martindale, Carrie W. Rinker-Schaeffer, Myriam Gorospe*

*To whom correspondence should be addressed. E-mail: myriam-gorospe{at}

This PDF file includes:

  • Methods
  • Reference
  • Fig. S1. MKK4 abundance increases with aging in a panel of tissue biopsies from young and old individual donors.
  • Fig. S2. Survey of putative MKK4 mRNA-interacting RNA-binding proteins.
  • Fig. S3. Higher expression of predicted human MKK4 mRNA-directed miRNAs in fetal tissues compared with adult tissues.
  • Fig. S4. Predicted microRNA-MKK4 mRNA hybrids.
  • Fig. S5. Unchanged MKK7 and Hsp27 abundance despite changes in MKK4.
  • Fig. S6. Point mutations introduced at each predicted miRNA site.
  • Fig. S7. MKK4 reporter analysis in HeLa cells recapitulates MKK4 reporter analysis in WI-38 HDFs.
  • Fig. S8. Activities of caspase-3 and caspase-7 remain unaltered after modulating MKK4.
  • Fig. S9. Analysis of cells treated with (Pre)miRNAs and (AS)miRNAs to modulate MKK4 reveals that MKK4 does not appear to activate the JNK pathway in S cells.

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Citation: B. S. Marasa, S. Srikantan, K. Masuda, K. Abdelmohsen, Y. Kuwano, X. Yang, J. L. Martindale, C. W. Rinker-Schaeffer, M. Gorospe, Increased MKK4 Abundance with Replicative Senescence Is Linked to the Joint Reduction of Multiple MicroRNAs. Sci. Signal. 2, ra69 (2009).

© 2009 American Association for the Advancement of Science

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