The Rfx4 Transcription Factor Modulates Shh Signaling by Regional Control of Ciliogenesis

Amir M. Ashique, Youngshik Choe, Mattias Karlen, Scott R. May, Khanhky Phamluong, Mark J. Solloway, Johan Ericson, Andrew S. Peterson*

*To whom correspondence should be addressed. E-mail: peterson.andrew{at}gene.com

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• Fig. S1. The Rfx4^L298P mutant brain phenotype displays a range of severity.
• Fig. S2. Dorsal midline defects in the developing telencephalon of Rfx4^L298P mutants.
• Fig. S3. Rfx1 and Rfx2 localize to the nucleus and Rfx4 is phosphorylated on a tyrosine residue.
• Fig. S4. Dorsal expansion of Shh signaling in the telencephalon of Rfx4^L298P mutants is consistent along the rostral-caudal axis.
• Fig. S5. Cilia structure defects in the dorsal telencephalon of Rfx4^L298P mutants.
• Fig. S6. Sample of SEM images used for cilia measurements.
• Fig. S7. Ift172 expression is decreased in Rfx4^L298P mutants.
• Fig. S8. Schematic of endogenous Rfx4 function and the block in Rfx4 function in the mutant Rfx4^L298P phenotype.
• Fig. S9. Rfx1, 2, and 3 expression during midgestation.
• Fig. S10. Digit patterning is normal in Rfx4^L298P mutants.

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Citation: A. M. Ashique, Y. Choe, M. Karlen, S. R. May, K. Phamluong, M. J. Solloway, J. Ericson, A. S. Peterson, The Rfx4 Transcription Factor Modulates Shh Signaling by Regional Control of Ciliogenesis. Sci. Signal. 2, ra70 (2009).

© 2009 American Association for the Advancement of Science