Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 3 November 2009
[DOI: 10.1126/scisignal.2000602]

Supplementary Materials for:

The Rfx4 Transcription Factor Modulates Shh Signaling by Regional Control of Ciliogenesis

Amir M. Ashique, Youngshik Choe, Mattias Karlen, Scott R. May, Khanhky Phamluong, Mark J. Solloway, Johan Ericson, Andrew S. Peterson*

*To whom correspondence should be addressed. E-mail: peterson.andrew{at}gene.com

This PDF file includes:

  • Fig. S1. The Rfx4L298P mutant brain phenotype displays a range of severity.
  • Fig. S2. Dorsal midline defects in the developing telencephalon of Rfx4L298P mutants.
  • Fig. S3. Rfx1 and Rfx2 localize to the nucleus and Rfx4 is phosphorylated on a tyrosine residue.
  • Fig. S4. Dorsal expansion of Shh signaling in the telencephalon of Rfx4L298P mutants is consistent along the rostral-caudal axis.
  • Fig. S5. Cilia structure defects in the dorsal telencephalon of Rfx4L298P mutants.
  • Fig. S6. Sample of SEM images used for cilia measurements.
  • Fig. S7. Ift172 expression is decreased in Rfx4L298P mutants.
  • Fig. S8. Schematic of endogenous Rfx4 function and the block in Rfx4 function in the mutant Rfx4L298P phenotype.
  • Fig. S9. Rfx1, 2, and 3 expression during midgestation.
  • Fig. S10. Digit patterning is normal in Rfx4L298P mutants.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2,367 KB


Citation: A. M. Ashique, Y. Choe, M. Karlen, S. R. May, K. Phamluong, M. J. Solloway, J. Ericson, A. S. Peterson, The Rfx4 Transcription Factor Modulates Shh Signaling by Regional Control of Ciliogenesis. Sci. Signal. 2, ra70 (2009).

© 2009 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882