A Loss-of-Function Screen Reveals Ras- and Raf-Independent MEK-ERK Signaling During Chlamydia trachomatis Infection

Rajendra Kumar Gurumurthy, André P. Mäurer, Nikolaus Machuy, Simone Hess, Klaus P. Pleissner, Johannes Schuchhardt, Thomas Rudel, Thomas F. Meyer*

*To whom correspondence should be addressed. E-mail: meyer{at}mpiib-berlin.mpg.de

This PDF file includes:

• Fig. S1. Overview of the image analysis with Scan R analysis software.
• Fig. S2. siRNA controls and plate layout.
• Fig. S3. Correlation plot for platewise quality control.
• Fig. S4. Two-step normalization for POC analysis.
• Fig. S5. Images for validated hits.
• Fig. S6. Infectivity phenotypes upon knockdown of Ras and Raf family members.
• Descriptions of supplementary tables.

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Technical Details

Format: Adobe Acrobat PDF

Size: 2.7 MB

Other Supplementary Material for this manuscript includes the following:

• Table S1. Primary screen data list (Excel file).
• Table S2. Hit validation data list (Excel file).
• Table S3. Final hit list (Excel file).
• Table S4. Sequences of siRNAs used in the primary screen (Excel file).
• Table S5. Sequences of the siRNAs used to validate the hits (Excel file).

.[Download Tables S1 to S5 (Compressed)]

Technical Details

Format: Microsoft Excel

Size: 1.1 MB (compressed); 2.8 MB (decompressed)

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© 2010 American Association for the Advancement of Science