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Sci. Signal., 13 April 2010
[DOI: 10.1126/scisignal.2000594]

Supplementary Materials for:

Identification of the miR-106b~25 MicroRNA Cluster as a Proto-Oncogenic PTEN-Targeting Intron That Cooperates with Its Host Gene MCM7 in Transformation

Laura Poliseno, Leonardo Salmena, Luisa Riccardi, Alessandro Fornari, Min Sup Song, Robin M. Hobbs, Paolo Sportoletti, Shorheh Varmeh, Ainara Egia, Giuseppe Fedele, Lucia Rameh, Massimo Loda, Pier Paolo Pandolfi*

*To whom correspondence should be addressed. E-mail: ppandolf{at}bidmc.harvard.edu

This PDF file includes:

  • Fig. S1. Direct interaction of miR-17, 19, 22, 25, and 302 with PTEN 3′UTR.
  • Fig. S2. miR-17, 19, 22, 25, and 302 families cause a decrease in the abundance of PTEN mRNA.
  • Fig. S3. Cooperation between different miRNAs in decreasing PTEN abundance.
  • Fig. S4. Score categories for in situ hybridization of miRNAs.
  • Fig. S5. The concomitant presence of miR-22, miR-25, miR-93, and miR-106b increases the probability of Akt phosphorylation.
  • Fig. S6. Analysis of DU145 stably infected with members of the miR-106b~25 cluster.
  • Fig. S7. sh-Pten partially mimics miR-22 and miR-106b~25 cluster.
  • Fig. S8. Constructs for expressing human MCM7 and the intronic miR-106b~25 cluster.
  • Fig. S9. Transgene expression in the Pb/MCM7i13 lines.
  • Fig. S10. MCM7 cannot initiate prostate tumorigenesis.
  • Fig. S11. Detection of miR-17~92 miRNAs by ISH in prostate tumor samples.
  • Table S1. Criteria used by four different prediction algorithms.
  • Table S2. Criterion 1 and/or Criterion 2 are fulfilled by seven miRNA families.
  • Table S3. Clinical and pathological characteristics of the patients.
  • Table S4. Correlation between miRNA up-regulation and DICER overexpression in prostate tumor samples.
  • Table S5. Primers used to generate chimeric luciferase plasmids.
  • Table S6. Primers used to generate PIG/22 and PIG/106b~25 plasmids.
  • Table S7. Primers used to detect human PTEN 3′UTR by PCR.
  • Table S8. Primers used to detect human PTEN, MCM7 and intron 13 mRNA by realtime PCR.
  • Table S9. Primers used to detect mouse Pten by real-time PCR.

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Citation: L. Poliseno, L. Salmena, L. Riccardi, A. Fornari, M. S. Song, R. M. Hobbs, P. Sportoletti, S. Varmeh, A. Egia, G. Fedele, L. Rameh, M. Loda, P. P. Pandolfi, Identification of the miR-106b~25 MicroRNA Cluster as a Proto-Oncogenic PTEN-Targeting Intron That Cooperates with Its Host Gene MCM7 in Transformation. Sci. Signal. 3, ra29 (2010).

© 2010 American Association for the Advancement of Scienceï


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