SUMOylation of the Transcriptional Co-Repressor KAP1 Is Regulated by the Serine and Threonine Phosphatase PP1

Xu Li, H. Helen Lin, Hanqing Chen, Xingzhi Xu, Hsiu-Ming Shih, David K. Ann*

*To whom correspondence should be addressed. E-mail: dann{at}coh.org

This PDF file includes:

• Methods
• Fig. S1. Tetracycline-inducible knockdown of KAP1 decreases the proliferation of MCF-7 cells.
• Fig. S2. The F370A and F370G mutations have opposing effects on the interactions of KAP1 with PP1α.
• Fig. S3. The S440/501A and S440/501D double mutations of KAP1 do not affect its interaction with PP1α or PP1β or the predicted secondary structure of the PP1-docking motif.
• Fig. S4. The F370G mutation of KAP1 attenuates the PP1α-dependent dephosphorylation of pSer⁸²⁴.
• Fig. S5. PP1α and PP1β and the regulation of KAP1 Ser⁸²⁴ phosphorylation, RanGAP1 SUMOylation, and MCF-7 cell proliferation.
• Fig. S6. PP1α and PP1β do not occupy the −713 and −3038 distal regions of p21 and they fail to affect the modification of K9 in histone H3 at the −3038 distal region.
• Table S1. Primer pairs used in real-time PCR assays.
• References

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