Evolution of CASK into a Mg²⁺-Sensitive Kinase

Konark Mukherjee,* Manu Sharma, Reinhard Jahn, Markus C. Wahl,* Thomas C. Südhof*

*To whom correspondence should be addressed. E-mail: konark{at}brandeis.edu (K.M.); mwahl{at}chemie.fu-berlin.de (M.C.W.); tcs1{at}stanford.edu (T.C.S.)

This PDF file includes:

• Fig. S1. Specificity of TNP-ATP binding.
• Fig. S2. Autophosphorylation of wild-type (WT) and mutant CASK CaMK domains.
• Fig. S3. TNP-ATP binding.
• Fig. S4. Autophosphorylation of the CASK^4M CaMK domain in the presence of Mg²⁺.
• Fig. S5. Stereo plots showing close-ups of the nucleotide-binding pocket of CASK^4M CaMK.
• Fig. S6. Regulatory segment of the CASK CaMK domain.
• Fig. S7. Sequence alignment of CASK orthologs.

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