Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 4 May 2010
[DOI: 10.1126/scisignal.2000796]

Supplementary Materials for:

Loops Govern SH2 Domain Specificity by Controlling Access to Binding Pockets

Tomonori Kaneko, Haiming Huang, Bing Zhao, Lei Li, Huadong Liu, Courtney K. Voss, Chenggang Wu, Martin R. Schiller, Shawn Shun-Cheng Li*

*To whom correspondence should be addressed. E-mail: sli{at}uwo.ca

This PDF file includes:

  • Fig. S1. Representative SH2 domain structures alone or in complex with peptides.
  • Fig. S2. Stereo representation of the crystal structure of the BRDG1 SH2 domain–NTAL complex.
  • Fig. S3. Contact area per residue of the NTAL pTyr136 peptide with the BRDG1 SH2 domain.
  • Fig. S4. The conserved hydrogen bonding network at P+1.
  • Fig. S5. Structure-based sequence alignment of a nonredundant group of 63 SH2 domains.
  • Fig. S6. Specificity switch by loop mutagenesis.
  • Fig. S7. Schematic description of the binding pockets in the Fyn SH2 domain and the loop-deletion mutants.
  • Table S1. Crystallographic statistics.
  • Table S2. Dissociation constants of SH2-peptide interactions measured by fluorescence polarization.
  • Table S3. Peptide binding assays using different BRDG1 SH2 domain constructs.
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 5.5 MB

Other Supplementary Material for this manuscript includes the following:

  • Interactive figures. JMOL representations of the structures shown in Fig. 3A.

.[View The Interactive Figures]


Citation: T. Kaneko, H. Huang, B. Zhao, L. Li, H. Liu, C. K. Voss, C. Wu, M. R. Schiller, S. S.-C. Li, Loops Govern SH2 Domain Specificity by Controlling Access to Binding Pockets. Sci. Signal. 3, ra34 (2010).

© 2010 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882