Loops Govern SH2 Domain Specificity by Controlling Access to Binding Pockets (Interactive Figure 3A)

Tomonori Kaneko, Haiming Huang, Bing Zhao, Lei Li, Huadong Liu, Courtney K. Voss, Chenggang Wu, Martin R. Schiller, Shawn Shun-Cheng Li*

*To whom correspondence should be addressed. E-mail: sli{at}uwo.ca

Interactive image 1. Surface representation of the SH2 domain of Grb2 bound to a peptide. This structure is based on PDB entry 1JYR and visualized with JMOL (1). The EF and BG loops are shown in brown and magenta, respectively. The EF loop residue W121 is depicted in cyan. The peptide ligand is shown as a stick representation. This is an interactive version of the Grb2 molecular structure shown in Figure 3. The static image is the same one that appears in the full text.

Interactive image 2. Surface representation of the SH2 domain of NCK2 bound to a peptide. This structure is based on PDB entry 2CIA and visualized with JMOL (1). The EF and BG loops are shown in brown and magenta, respectively. The peptide ligand is shown as a stick representation. This is an interactive version of the NCK2 molecular structure shown in Figure 3. The static image is the same one that appears in the full text.

Interactive image 3. Surface representation of the SH2 domain of BRDG1 bound to a peptide. This structure is based on PDB entry 3MAZ and visualized with JMOL (1). The EF and BG loops are shown in brown and magenta, respectively. The EF loop residue L240 is depicted in cyan. Residues lining or encompassing the pocket, including Ile²¹⁵ (βC6), Tyr²²⁷ (βD5), Ile²³⁸ (βE4), Tyr²⁵⁵ (αB8), Phe²⁵⁶ (αB9), Glu²⁵⁹ (αB12), Thr²⁶⁰ (BG1), and Leu²⁶⁴ (the leucine anchor), are colored orange. The peptide ligand is shown as a stick representation. This is an interactive version of the BRDG1 molecular structure shown in Figure 3. The static image is the same one that appears in the full text.

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Reference

1. Jmol: An open-source Java viewer for chemical structures in 3D. http://www.jmol.org/.

Citation: T. Kaneko, H. Huang, B. Zhao, L. Li, H. Liu, C. K. Voss, C. Wu, M. R. Schiller, S. S.-C. Li, Loops govern SH2 domain specificity by controlling access to binding pockets. Sci. Signal. 3, ra34 (2010).