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Sci. Signal., 18 May 2010
[DOI: 10.1126/scisignal.2000500]

Supplementary Materials for:

TREM2- and DAP12-Dependent Activation of PI3K Requires DAP10 and Is Inhibited by SHIP1

Qisheng Peng, Shikha Malhotra, James A. Torchia, William G. Kerr, K. Mark Coggeshall, Mary Beth Humphrey*

*To whom correspondence should be addressed. E-mail: marybeth-humphrey{at}ouhsc.edu

This PDF file includes:

  • Fig. S1. Recruitment of PLC-γ2 and Grb2 to DAP12 occurs upon ligation of TREM2.
  • Fig. S2. DAP10 and DAP12 form homodimers and heterodimers in macrophages.
  • Fig. S3. DAP10 is not required for the expression of TREM2 at the cell surface or for TREM2-induced Ca2+ flux.
  • Fig. S4. Ligation of TREM2 leads to protection from apoptosis by inducing the
    production of MCL-1.
  • Fig. S5. Inhibition of PI3K, but not of Src or Syk, increases the amount of SHIP1 recruited to DAP12.
  • Fig. S6. SHIP1 and DAP10 colocalize with actin in cap-like structures after ligation
    of TREM2.
  • Fig. S7. DAP10 is not required for the translocation of SHIP1 to the plasma
    membrane.

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Citation: Q. Peng, S. Malhotra, J. A. Torchia, W. G. Kerr, K. M. Coggeshall, M. B. Humphrey, TREM2- and DAP12-Dependent Activation of PI3K Requires DAP10 and Is Inhibited by SHIP1. Sci. Signal. 3, ra38 (2010).

© 2010 American Association for the Advancement of Science


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