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Sci. Signal., 18 May 2010
[DOI: 10.1126/scisignal.2000678]

Supplementary Materials for:

c-mip Impairs Podocyte Proximal Signaling and Induces Heavy Proteinuria

Shao-yu Zhang, Maud Kamal, Karine Dahan, André Pawlak, Virginie Ory, Dominique Desvaux, Vincent Audard, Marina Candelier, Fatima Ben Mohamed, Marie Matignon, Christo Christov, Xavier Decrouy, Veronique Bernard, Gilles Mangiapan, Philippe Lang, Georges Guellaën, Pierre Ronco, Djillali Sahali*

*To whom correspondence should be addressed. E-mail: dil.sahali{at}inserm.fr

This PDF file includes:

  • Fig. S1. Representative in situ hybridization of serial sections from kidney biopsies from patients with MN and FSGS.
  • Fig. S2. Detection of c-mip in other glomerular diseases.
  • Fig. S3. Generation of c-mip transgenic mice.
  • Fig. S4. PAS staining of kidney sections from 8-week-old wild-type and proteinuric Tg(+) mice.
  • Fig. S5. Morphological characterization of c-mip transgenic mice.
  • Fig. S6. Quantification of confocal microscopy analysis of c-mip abundance in glomeruli from MCNS kidney biopsies and renal tissue of Tg(+) mice.
  • Fig. S7. Ultrastructural analysis of podocytes from 5-day-old newborn transgenic mice.
  • Fig. S8. Course of glomerular disease in Tg(+) mice.
  • Fig. S9. Confocal microscopy analysis of the abundance of total and phosphorylated nephrin in kidney sections from 8-week-old wild-type and Tg(+) mice.
  • Fig. S10. Quantitation of confocal microscopy analysis of phosphorylation of Ser473 in Akt in glomerular diseases.
  • Fig. S11. Specificity of the c-mip antibody.
  • Fig. S12. Interaction of c-mip with Fyn and N-WASP.
  • Fig. S13. Immunogold labeling showing c-mip localization in primary and secondary podocyte foot processes in LPS-treated mice.
  • Table S1. Patient characteristics at the time of biopsy.
  • Table S2. Quantification of c-mip abundance in glomeruli of kidney tissues from MCNS patients and Tg(+) mice.
  • Table S3. Semiquantitative measurement of urinary protein concentration in newborn transgenic mice.
  • Table S4. Sequence of primers and PCR conditions.

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Citation: S.-y. Zhang, M. Kamal, K. Dahan, A. Pawlak, V. Ory, D. Desvaux, V. Audard, M. Candelier, F. B. Mohamed, M. Matignon, C. Christov, X. Decrouy, V. Bernard, G. Mangiapan, P. Lang, G. Guellaën, P. Ronco, D. Sahali, c-mip Impairs Podocyte Proximal Signaling and Induces Heavy Proteinuria. Sci. Signal. 3, ra39 (2010).

© 2010 American Association for the Advancement of Science


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