Nodal Signaling Recruits the Histone Demethylase Jmjd3 to Counteract Polycomb-Mediated Repression at Target Genes

Øyvind Dahle, Amit Kumar, Michael R. Kuehn*

*To whom correspondence should be addressed. E-mail: mkuehn{at}mail.nih.gov

This PDF file includes:

• Fig. S1. Quantification of the abundance of Nodal mRNA in WT ES cells and in B16
  melanoma cells.
• Fig. S2. ChIP analysis of Smads2/3 at the PEE and ASE regions of the Nodal locus.
• Fig. S3. The abundance of total histone H3 at the Nodal and Brachyury loci.
• Fig. S4. Changes in Nodal expression and chromatin status after 24 hours of
  signaling inhibition.
• Fig. S5. Expression of Nanog and Oct4 after knockdown of Jmjd3.
• Table S1. Primers used for RT-PCR analysis.
• Table S2. Primers used for ChIP analysis.

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