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Sci. Signal., 27 July 2010
[DOI: 10.1126/scisignal.2000810]

Supplementary Materials for:

Decoupling of Receptor and Downstream Signals in the Akt Pathway by
Its Low-Pass Filter Characteristics

Kazuhiro A. Fujita, Yu Toyoshima, Shinsuke Uda, Yu-ichi Ozaki, Hiroyuki Kubota, Shinya Kuroda*

*To whom correspondence should be addressed. E-mail: skuroda{at}

This PDF file includes:

  • Fig. S1. Gel images of immunoblots.
  • Fig. S2. Akt pathway model.
  • Fig. S3. Dose responses of pEGFR, pAkt, and pS6.
  • Fig. S4. EGF-dependent phosphorylation of S6K.
  • Fig. S5. Inhibition of S6 phosphorylation by LY294002 or rapamycin.
  • Fig. S6. Concept of frequency response analysis.
  • Fig. S7. Frequency response analysis of the Akt pathway model.
  • Fig. S8. Responses of the fitted consecutive reactions.
  • Fig. S9. NGF-dependent Akt pathway.
  • Fig. S10. NGF-dependent Akt pathway model.
  • Fig. S11. Time courses of the low-, middle-, and high-frequency waves of pEGFR, pAkt, and pS6.
  • Fig. S12. Inhibitor model.
  • Fig. S13. Parameter sensitivity analysis of EGF-dependent Akt pathway model.

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Other Supplementary Material for this manuscript includes the following:

  • Model files in COPASI (.cps) and SBML (.xml) format for the EGF-Akt pathway model, the NGF-Akt pathway model, and the inhibitor pathway model.

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Citation: K. A. Fujita, Y. Toyoshima, S. Uda, Y.-i. Ozaki, H. Kubota, S. Kuroda, Decoupling of Receptor and Downstream Signals in the Akt Pathway by Its Low-Pass Filter Characteristics. Sci. Signal. 3, ra56 (2010).

© 2010 American Association for the Advancement of Science

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