The Drosophila Female Germline Stem Cell Lineage Acts to Spatially
Restrict DPP Function Within the Niche

Ming Liu, Tit Meng Lim, Yu Cai*

*To whom correspondence should be addressed. E-mail: caiyu{at}tll.org.sg

This PDF file includes:

• Fig. S1. The generation of the stet^1A3 mutant.
• Fig. S2. stet functions in the germ line and is involved in the generation of multiple
  EGFR ligands.
• Fig. S3. EGFR functions through the MAPK but not the PI3K or PLC-γ pathway.
• Fig. S4. pERK is expressed in ESCs and ECs.
• Fig. S5. stet^1A3 mutant germarium exhibits non–cell autonomous defects.
• Fig. S6. stet is required in GSCs for germline homeostasis.
• Fig. S7. pMad expression domain is expanded in stet^1A3 germaria.
• Fig. S8. Dad-lacZ expression domain is expanded in EGFR-MAPK germaria.
• Fig. S9. dpp transcripts are detected in wild-type and stet^1A3 germaria.
• Fig. S10. DPP functions in the niche.
• Fig. S11. dally mRNA is detected in wild-type and EGFR-MAPK germaria.
• Fig. S12. A model.
• Fig. S13. Defective cellular extension is not sufficient for expanded DPP function
  outside the niche.
• Fig. S14. Germaria with ectopic Hh or Wg expression do not exhibit the formation of
  ectopic spectrosome-containing cells.
• Fig. S15. Conditional removal of DPP from the niche suppresses the stet^1A3 mutant
  phenotype.

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