Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 17 August 2010
[DOI: 10.1126/scisignal.2000955]

Supplementary Materials for:

Arginine Usage in Mycobacteria-Infected Macrophages Depends on Autocrine-Paracrine Cytokine Signaling

Joseph E. Qualls, Geoffrey Neale, Amber M. Smith, Mi-Sun Koo, Ashley A. DeFreitas, Huiyuan Zhang, Gilla Kaplan, Stephanie S. Watowich, Peter J. Murray*

*To whom correspondence should be addressed. E-mail: peter.murray{at}stjude.org

This PDF file includes:

  • Materials and Methods
  • Fig. S1. The expression of Arg1 and C/EBPβ during infection with BCG is dependent on MyD88.
  • Fig. S2. Principal components analysis (PCA) of the Affymetrix transcript array.
  • Fig. S3. C/EBPβ is partially required for the expression of Arg1 after stimulation with supernatant from BCG-infected cells.
  • Fig. S4. BCG-induced expression of Arg1 is independent of the alternative activation of macrophages.
  • Fig. S5. IL-6, IL-10, and G-CSF are produced by wild-type, but not Myd88–/–, macrophages after infection with BCG.
  • Fig. S6. Expression of Arg1 is induced by recombinant IL-6, IL-10, and G-CSF.
  • Fig. S7. The expression of Arg1 is intact in BMDMs from Il6–/– mice after infection with BCG.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 735 KB


Citation: J. E. Qualls, G. Neale, A. M. Smith, M.-S. Koo, A. A. DeFreitas, H. Zhang, G. Kaplan, S. S. Watowich, P. J. Murray, Arginine usage in mycobacteria-infected macrophages depends on autocrine-paracrine cytokine signaling. Sci. Signal. 3, ra62 (2010).

© 2010 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882