Arginine Usage in Mycobacteria-Infected Macrophages Depends on Autocrine-Paracrine Cytokine Signaling

Joseph E. Qualls, Geoffrey Neale, Amber M. Smith, Mi-Sun Koo, Ashley A. DeFreitas, Huiyuan Zhang, Gilla Kaplan, Stephanie S. Watowich, Peter J. Murray*

*To whom correspondence should be addressed. E-mail: peter.murray{at}stjude.org

This PDF file includes:

• Materials and Methods
• Fig. S1. The expression of Arg1 and C/EBPβ during infection with BCG is dependent on MyD88.
• Fig. S2. Principal components analysis (PCA) of the Affymetrix transcript array.
• Fig. S3. C/EBPβ is partially required for the expression of Arg1 after stimulation with supernatant from BCG-infected cells.
• Fig. S4. BCG-induced expression of Arg1 is independent of the alternative activation of macrophages.
• Fig. S5. IL-6, IL-10, and G-CSF are produced by wild-type, but not Myd88^–/–, macrophages after infection with BCG.
• Fig. S6. Expression of Arg1 is induced by recombinant IL-6, IL-10, and G-CSF.
• Fig. S7. The expression of Arg1 is intact in BMDMs from Il6^–/– mice after infection with BCG.

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