Signaling from the Endoplasmic Reticulum Activates Brassinosteroid Signaling and Promotes Acclimation to Stress in Arabidopsis

Ping Che,* John D. Bussell, Wenxu Zhou, Gonzalo M. Estavillo, Barry J. Pogson, Steven M. Smith*

*To whom correspondence should be addressed. E-mail: ssmith{at}cyllene.uwa.edu.au (S.M.S.); pche{at}cyllene.uwa.edu.au (P.C.)

This PDF file includes:

• Fig. S1. A diagram of regulated intramembrane proteolysis.
• Fig. S2. Localization of S1P, S2P, and bZIP28.
• Fig. S3. Characterization of s2p mutant.
• Fig. S4. Structures of full-length and truncated bZIPs.
• Fig. S5. S2P-RIP does not affect BR biosynthesis gene expression.
• Fig. S6. BES1 does not induce chaperone gene expression in wild-type or s2p backgrounds.
• Fig. S7. Possible interactions of stress-induced S2P-RIP with BR signaling pathway.
• Table S3. Oligonucleotides used for PCR, RT-PCR, and qRT-PCR.
• Table S4. Oligonucleotides used for plasmid constructs.

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Other Supplementary Material for this manuscript includes the following:

• Table S1. Complementation of bri1-5 down-regulated genes by bZIPΔC (Microsoft Excel format).
• Table S2. Complementation of bri1-5 up-regulated genes by bZIPΔCs (Microsoft Excel format).

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