Sexually Dimorphic Actions of Glucocorticoids Provide a Link to Inflammatory Diseases with Gender Differences in Prevalence

Danielle Duma, Jennifer B. Collins, Jeff W. Chou, John A. Cidlowski*

*To whom correspondence should be addressed. E-mail: cidlows1{at}niehs.nih.gov

This PDF file includes:

• Methods
• Fig. S1. Nascent RNA analysis of glucocorticoid-regulated genes.
• Fig. S2. Comparison of male and female rat liver cross sections stained with hematoxylin and eosin.
• Fig. S3. Comparison of glucocorticoid receptor mRNA and protein abundance and binding to [³H]dexamethasone in male and female rat liver.
• Fig. S4. Comparison of glucocorticoid receptor mRNA and protein abundance in the liver of female rats in different phases of estrous cycle.
• Fig. S5. Sexually dimorphic regulation of gene expression by glucocorticoids in isolated hepatocytes.
• Fig. S6. Sexually dimorphic regulation of gene expression by glucocorticoids in mouse liver.
• Fig. S7. Analyses of gender-specific expression patterns of nuclear receptor coregulators.
• Fig. S8. Analysis of dose-response curves for dexamethasone in male and female rat livers.
• References

[Download Corrected PDF]

CORRECTED 26 OCTOBER 2010: The authors inadvertently included the wrong graph for panel B of Figure S8 of the Supplementary Materials. In the legend, the graph was described as showing the mRNA abundance of FkBp5, but graph showed that of Gucy2c.

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The original, uncorrected version can be downloaded here.

Other Supplementary Material for this manuscript includes the following:

• Table S1. List of glucocorticoid-responsive genes in male and female rat liver for each pattern of gene expression identified by EPIG analyses (Microsoft Excel format).
• Table S2. List of genes contained in gene networks generated by IPA for each pattern of gene expression (Microsoft Excel format).
• Table S3. List of common, male-specific, and female-specific glucocorticoidregulated genes involved in inflammatory disorders (Microsoft Excel format).
• Table S4. List of gender-specific nuclear receptor co-regulators (Microsoft Excel format).

© 2010 American Association for the Advancement of Science