Antagonistic Regulation of Actin Dynamics and Cell Motility by TRPC5 and TRPC6 Channels

Dequan Tian, Sarah M. P. Jacobo, David Billing, Anete Rozkalne, Steven D. Gage, Theodora Anagnostou, Hermann Pavenstaedt, Hsiang-Hao Hsu, Johannes Schlondorff, Arnolt Ramos, Anna Greka*

*To whom correspondence should be addressed. E-mail: greka.anna{at}mgh.harvard.edu

This PDF file includes:

• Fig. S1. Ang II–evoked TRPC-like currents in podocytes.
• Fig. S2. Gene silencing of TRPC channels in a lentiviral delivery system.
• Fig. S3. Ca²⁺ imaging and patch-clamp electrophysiology in TRPC-depleted podocytes.
• Fig. S4. Ang II–induced TRPC5 and TRPC6 activation has antagonistic effects on the actin cytoskeleton in wild-type podocytes.
• Fig. S5. TRPC gene silencing in podocytes and antibody validation.
• Fig. S6. Colocalization of TRPC5 with Rac1 and TRPC6 with RhoA in podocytes.
• Fig. S7. Rac1 mediates increased Ang II–dependent TRPC5 insertion into the plasma membrane.
• Fig. S8. Podocytes at the edge of the wound display characteristic actin phenotypes.

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© 2010 American Association for the Advancement of Science