Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 26 October 2010
[DOI: 10.1126/scisignal.2000738]

Supplementary Materials for:

Regulation of 3-Phosphoinositide–Dependent Protein Kinase 1 Activity by Homodimerization in Live Cells

Thomas A. Masters, Véronique Calleja, Daven A. Armoogum, Richard J. Marsh, Christopher J. Applebee, Michel Laguerre, Angus J. Bain,* Banafshé Larijani*

*To whom correspondence should be addressed. E-mail: banafshe.larijani{at}cancer.org.uk (B.L.); a.bain{at}ucl.ac.uk (A.J.B.)

This PDF file includes:

  • Methods
  • Fig. S1. Coimmunoprecipitation of myc-PDK1 with GFP-myc-PDK1 from COS-7 cells.
  • Fig. S2. In vitro phosphorylation of the PDKtide peptide by recombinant tagged PDK1.
  • Fig. S3. Analysis of sensitized acceptor fluorescence.
  • Fig. S4. Molecular modeling of the PDK1 homodimer.
  • Fig. S5. Example of the calculation of pixel enrichment.
  • Fig. S6. Probabilities that a PDK1 dimer can be detected by hetero-FRET.
  • Reference

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 423 KB


Citation: T. A. Masters, V. Calleja, D. A. Armoogum, R. J. Marsh, C. J. Applebee, M. Laguerre, A. J. Bain, B. Larijani, Regulation of 3-Phosphoinositide–Dependent Protein Kinase 1 Activity by Homodimerization in Live Cells. Sci. Signal. 3, ra78 (2010).

© 2010 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882