Activation of STIM1-Orai1 Involves an Intramolecular Switching Mechanism

Marek K. Korzeniowski, Isabel Martín Manjarrés, Peter Varnai, Tamas Balla*

*To whom correspondence should be addressed. E-mail: ballat{at}mail.nih.gov

This PDF file includes:

• Fig. S1. Clustering of STIM1 cytosolic fragment in rapamycin-induced mito-PM contact zones activates Ca²⁺ entry.
• Fig. S2. Clustering of Orai1 is not sufficient to increase cytosolic Ca²⁺ concentration.
• Fig. S3. Inhibition of STIM1-ct–induced Ca²⁺ influx by 2-APB in COS-7 cells.
• Fig. S4. Morphology of TK-driven YFP-tagged STIM1 mutant proteins in COS-7 cells without expression of Orai1.
• Fig. S5. Western analysis showing the expression and integrity of the various STIM1-ct forms in both their recruitable (FKBP12-fused) and nonrecruitable versions.
• Fig. S6. Effect of linker length on the ability of STIM1-ct to activate Orai1-mediated Ca²⁺ influx.
• Table S1. PCR primers used to amplify the segments cloned in the various constructs.

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