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Sci. Signal., 23 November 2010
[DOI: 10.1126/scisignal.2001148]

Supplementary Materials for:

BRAF Gene Amplification Can Promote Acquired Resistance to MEK Inhibitors in Cancer Cells Harboring the BRAF V600E Mutation

Ryan B. Corcoran, Dora Dias-Santagata, Kristin Bergethon, A. John Iafrate, Jeffrey Settleman,* Jeffrey A. Engelman*

*To whom correspondence should be addressed. E-mail: Settleman.jeffrey{at}gene.com (J.S.); jengelman{at}partners.org (J.A.E.)

This PDF file includes:

  • Fig. S1. AR cells are resistant to multiple MEK and BRAF inhibitors.
  • Fig. S2. The ability of AZD6244 and AZ628 to inhibit ERK phosphorylation is attenuated in AR cells.
  • Fig. S3. CRAF is not amplified in AR cells.
  • Fig. S4. Knockdown of BRAF, but not CRAF, restores the ability of AZD6244 to inhibit ERK phosphorylation.
  • Fig. S5. Resistance caused by overexpression of V600E BRAF can be overcome by combined MEK and BRAF inhibition.
  • Fig. S6. Enhanced inhibition of ERK phosphorylation underlies the increased sensitivity of parental cells to combination treatment with AZD6244 and AZ628.
  • Table S1. BRAF amplification in BRAF-mutant human colorectal cancer.

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Citation: R. B. Corcoran, D. Dias-Santagata, K. Bergethon, A. J. Iafrate, J. Settleman, J. A. Engelman, BRAF Gene Amplification Can Promote Acquired Resistance to MEK Inhibitors in Cancer Cells Harboring the BRAF V600E Mutation. Sci. Signal. 3, ra84 (2010).

© 2010 American Association for the Advancement of Science

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