Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 21 December 2010
[DOI: 10.1126/scisignal.2001423]

Supplementary Materials for:

Cyclic GMP and Protein Kinase G Control a Src-Containing
Mechanosome in Osteoblasts

Hema Rangaswami, Raphaela Schwappacher, Nisha Marathe, Shunhui Zhuang, Darren E. Casteel, Bodo Haas, Yong Chen, Alexander Pfeifer, Hisashi Kato, Sanford Shattil, Gerry R. Boss, Renate B. Pilz*

*To whom correspondence should be addressed. E-mail: rpilz{at}ucsd.edu

This PDF file includes:

  • Materials and Methods
  • Fig. S1. siRNA knockdown of PKGI or PKGII; effects of fluid shear stress and cGMP on Src and ERK phosphorylation in MLO-Y4 and MC3T3 cells.
  • Fig. S2. PKGII does not directly phosphorylate Src.
  • Fig. S3. Effect of vanadate- and phosphatase-specific siRNAs on Src phosphorylation and dephosphorylation.
  • Fig. S4. Analysis of SHP-1 phosphorylation and function in MC3T3 cells.
  • Fig. S5. cGMP activation of Src requires ligation of β3 integrins.
  • Fig. S6. Colocalization of β3 integrins, PKGII, and Src with SHP-2–containing membrane complexes.
  • Fig. S7. Interactions among PKGII, SHP-2, Src, and β3 integrins.
  • Table S1. siRNA target sequences.
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 847 KB


Citation: H. Rangaswami, R. Schwappacher, N. Marathe, S. Zhuang, D. E. Casteel, B. Haas, Y. Chen, A. Pfeifer, H. Kato, S. Shattil, G. R. Boss, R. B. Pilz, Cyclic GMP and Protein Kinase G Control a Src-Containing Mechanosome in Osteoblasts. Sci. Signal. 3, ra91 (2010).

© 2010 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882