Activation State of the M3 Muscarinic Acetylcholine Receptor Modulates Mammalian Odorant Receptor Signaling

Yun Rose Li and Hiroaki Matsunami*

*To whom correspondence should be addressed. E-mail: hiroaki.matsunami{at}duke.edu

This PDF file includes:

• Fig. S1. The M3-R increases the activation of untagged ORs in Hana3A cells.
• Fig. S2. The muscarinic acetylcholine receptor family members modulate OR signaling.
• Fig. S3. The M2-R is not detectable in the olfactory cilia.
• Fig. S4. Effects of carbachol, forskolin, and ionomycin on OR signaling.
• Fig. S5. Detailed response curves and response values of OSNs.
• Fig. S6. The M3-R does not affect the ligand specificity of ORs.
• Fig. S7. FLAG–OR-S6 is present at the cell surface when coexpressed with the M3-R and RTP1S.
• Fig. S8. FLAG–OR-S6 responds as robustly as Rho–OR-S6 and more robustly than untagged OR-S6.
• Fig. S9. Graphical explanation of the trapezoidal method.
• Fig. S10. The M3-R enhances the function of various mammalian ORs.
• Table S1. GPCRs tested for potentiation of OR signaling.
  

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