Genome-Wide RNAi Screen Reveals Disease-Associated Genes That Are Common to Hedgehog and Wnt Signaling

Leni S. Jacob, Xiaofeng Wu, Michael E. Dodge, Chih-Wei Fan, Ozlem Kulak, Baozhi Chen, Wei Tang, Baolin Wang, James F. Amatruda, Lawrence Lum*

*To whom correspondence should be addressed. E-mail: lawrence.lum{at}UTSouthwestern.edu

This PDF file includes:

• Fig. S1. Additional material relating to the RNAi screen.
• Fig. S2. Confirmatory siRNA assays for gene function in Hh pathway response.
• Fig. S3. Additional characterization of STK11^–/– cells.
• Fig. S4. Hh-induced Smo or Gli protein accumulation in the primary cilium is not affected in STK11^–/– cells.
• Fig. S5. Dvl phosphorylation is sensitive to IWP but not SAHA.
• Fig. S6. Dvl2 undergoes a change in distribution in response to Wnt protein stimulation in MEFs.
• Fig. S7. The defect in posterior morphogenesis and somite development is apparent in STK11 morphants stained for MyoD expression, which labels somitic tissue.
• Fig. S8. Loss of STK11 in cancerous cells induces Dvl protein phosphorylation and compromises primary cilia formation.
• Fig. S9. Cell line–dependent responses to Wnt ligand in cervical carcinoma cells lacking Stk11.
• Fig. S10. Axin1 forms punctate structures in HeLa cells.
  

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 255 KB

Other Supplementary Material for this manuscript includes the following:

• Table S1 (Microsoft Excel format). Dharmacon library primary screen results.
• Table S2 (Microsoft Excel format). Qiagen library primary screening results.
• Table S3 (Microsoft Excel format). Genes identified by two siRNA pools from the primary screen.
• Table S4 (Microsoft Excel format). Counterscreen results.
• Table S5 (Microsoft Excel format). siRNA sequences targeting disease genes.

© 2011 American Association for the Advancement of Science