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Sci. Signal., 8 February 2011
[DOI: 10.1126/scisignal.2001446]

Supplementary Materials for:

The Crystal Structure of a Self-Activating G Protein α Subunit Reveals Its Distinct Mechanism of Signal Initiation

Janice C. Jones, Jeffrey W. Duffy, Mischa Machius, Brenda R. S. Temple, Henrik G. Dohlman,* Alan M. Jones

*To whom correspondence should be addressed. E-mail: hdohlman{at}med.unc.edu

This PDF file includes:

  • Methods
  • Fig. S1. Solvent accessibility of guanine nucleotides in plant and animal G protein α subunits.
  • Fig. S2. B value analysis of AtGPA1.
  • Fig. S3. Nucleotide exchange by G protein α subunits.
  • Fig. S4. CD spectra of GDP-bound G protein α subunits.
  • Table S1. Data collection and refinement statistics for AtGPA1.
  • Table S2. GTP-binding rates for wild-type and mutant G protein α subunits.
  • Movie S1 legend.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 391 KB

Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). B value analysis of crystal structures of G protein α subunits. 67 KB
  • Movie S1 (.avi format). Movie depicting domain motion in AtGPA1 generated from MD simulations. 8.6 MB

Citation: J. C. Jones, J. W. Duffy, M. Machius, B. R. S. Temple, H. G. Dohlman, A. M. Jones, The Crystal Structure of a Self-Activating G Protein α Subunit Reveals Its Distinct Mechanism of Signal Initiation. Sci. Signal. 4, ra8 (2011).

© 2011 American Association for the Advancement of Science


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