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Sci. Signal., 8 March 2011
[DOI: 10.1126/scisignal.2001178]

Supplementary Materials for:

Vav1-Mediated Scaffolding Interactions Stabilize SLP-76 Microclusters and Contribute to Antigen-Dependent T Cell Responses

Nicholas R. Sylvain, Ken Nguyen, Stephen C. Bunnell*

*To whom correspondence should be addressed. E-mail: stephen.bunnell{at}tufts.edu

This PDF file includes:

  • Fig. S1. Effects of Vav1 mutants on TCR-induced Ca2+ flux.
  • Fig. S2. Mutations that affect the catalytic core of the Vav1 GEF.
  • Table S1. Effect of knockdown of Vav1 on SLP-76 microcluster dynamics.
  • Table S2. List of primers used to generate the various Vav1 mutations.
  • Movies S1 to S16 legend.

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Technical Details

Format: Adobe Acrobat PDF

Size: 322 KB

Other Supplementary Material for this manuscript includes the following:

  • Movies S1 to S16 (.avi format). Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Technical Details

Format: Quicktime movies in a zipped archive

Size: 9.42 MB


Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
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Citation: N. R. Sylvain, K. Nguyen, S. C. Bunnell, Vav1-Mediated Scaffolding Interactions Stabilize SLP-76 Microclusters and Contribute to Antigen-Dependent T Cell Responses. Sci. Signal. 4, ra14 (2011).

© 2011 American Association for the Advancement of Science


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