Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 15 March 2011
[DOI: 10.1126/scisignal.2001449]

Supplementary Materials for:

Engineering the ABA Plant Stress Pathway for Regulation of Induced Proximity

Fu-Sen Liang, Wen Qi Ho, Gerald R. Crabtree*

*To whom correspondence should be addressed. E-mail: crabtree{at}stanford.edu

This PDF file includes:

  • Fig. S1. Crystal structure of ABI1-(+)-ABA-PYL1 complex.
  • Fig. S2. Dose response of ABA-induced luciferase activation in cultured mammalian cell lines.
  • Fig. S3. Time course of luciferase activation by ABA or Rap in CHO cells.
  • Fig. S4. Reversibility of ABA- and Rap-induced luciferase production in E14 cells under different washing conditions.
  • Fig. S5. The amounts of fusion protein production in ABA and Rap system.
  • Fig. S6. Luciferase induction by ABA in different cell types.
  • Fig. S7. Distribution of GFP-PYLcs with or without ABA in HEK 293T cells.
  • Fig. S8. Quantification of cytoplasmic localization of GFP-PYLcs.
  • Fig. S9. ABA-induced nuclear localization of GFP-PYLcs.
  • Fig. S10. ABA-induced membrane localization of GFP-PYLcs.
  • Fig. S11. ABA-induced nuclear localization of Numb-GFP-PYLcs.
  • Fig. S12. Orthogonal induction of luciferase by ABA and Rap in NIH 3T3 cells.
  • Fig. S13. Independent ABA- or Rap-induced membrane localization of GFP fusion proteins.
  • Fig. S14. Independent relocalization of GFP and mCherry fusion proteins by ABA and Rap systems.
  • Fig. S15. Dose dependence of phosphatase activity of wild-type and mutant GST-ABIcs.
  • Fig. S16. Quantification of GFP-PYLcs pull-down by wild-type or mutant ABI.
  • Fig. S17. Luciferase activation by ABA-activator cassette with wild-type or mutant ABIcs in NIH 3T3 cells.
  • Fig. S18. ABA stability in FBS.
  • Fig. S19. Bioavailability of ABA in mice.
  • Fig. S20. Effect of ABA on lymphocyte proliferation.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.06 MB

 


Citation: F.-S. Liang, W. Q. Ho, G. R. Crabtree, Engineering the ABA Plant Stress Pathway for Regulation of Induced Proximity. Sci. Signal. 4, rs2 (2011).

© 2011 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882