Engineering the ABA Plant Stress Pathway for Regulation of Induced Proximity

Fu-Sen Liang, Wen Qi Ho, Gerald R. Crabtree*

*To whom correspondence should be addressed. E-mail: crabtree{at}stanford.edu

This PDF file includes:

• Fig. S1. Crystal structure of ABI1-(+)-ABA-PYL1 complex.
• Fig. S2. Dose response of ABA-induced luciferase activation in cultured mammalian cell lines.
• Fig. S3. Time course of luciferase activation by ABA or Rap in CHO cells.
• Fig. S4. Reversibility of ABA- and Rap-induced luciferase production in E14 cells under different washing conditions.
• Fig. S5. The amounts of fusion protein production in ABA and Rap system.
• Fig. S6. Luciferase induction by ABA in different cell types.
• Fig. S7. Distribution of GFP-PYL_cs with or without ABA in HEK 293T cells.
• Fig. S8. Quantification of cytoplasmic localization of GFP-PYL_cs.
• Fig. S9. ABA-induced nuclear localization of GFP-PYL_cs.
• Fig. S10. ABA-induced membrane localization of GFP-PYL_cs.
• Fig. S11. ABA-induced nuclear localization of Numb-GFP-PYL_cs.
• Fig. S12. Orthogonal induction of luciferase by ABA and Rap in NIH 3T3 cells.
• Fig. S13. Independent ABA- or Rap-induced membrane localization of GFP fusion proteins.
• Fig. S14. Independent relocalization of GFP and mCherry fusion proteins by ABA and Rap systems.
• Fig. S15. Dose dependence of phosphatase activity of wild-type and mutant GST-ABI_cs.
• Fig. S16. Quantification of GFP-PYL_cs pull-down by wild-type or mutant ABI.
• Fig. S17. Luciferase activation by ABA-activator cassette with wild-type or mutant ABI_cs in NIH 3T3 cells.
• Fig. S18. ABA stability in FBS.
• Fig. S19. Bioavailability of ABA in mice.
• Fig. S20. Effect of ABA on lymphocyte proliferation.
  

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.06 MB

© 2011 American Association for the Advancement of Science