Poly(ADP-Ribose) (PAR) Binding to Apoptosis-Inducing Factor Is Critical for PAR Polymerase-1–Dependent Cell Death (Parthanatos)

Yingfei Wang, No Soo Kim, Jean-Francois Haince, Ho Chul Kang, Karen K. David, Shaida A. Andrabi, Guy G. Poirier, Valina L. Dawson,* Ted M. Dawson*

*To whom correspondence should be addressed. E-mail: tdawson{at}jhmi.edu (T.M.D.); vdawson{at}jhmi.edu (V.L.D.)

This PDF file includes:

• Table S1. Primers used in sequential point mutation to construct single, double, triple, and quadruple point mutations.
• Fig. S1. PAR binds to AIF.
• Fig. S2. Alignment of the three putative PAR binding motifs in AIF.
• Fig. S3. Determination of amino acids in AIF responsible for PAR binding.
• Fig. S4. AIF and PAR binding affinity.
• Fig. S5. Subcellular localization of AIF and PAR in cortical neurons.
• Fig. S6. DNA and RNA are not involved in the PAR-AIF interaction.
• Fig. S7. Pbm-AIF fails to bind to PAR and is resistant to its release from mitochondria in HeLa cells.
• Fig. S8. Kinetics of AIF-mitochondria binding.
• Fig. S9. Pbm-AIF is resistant to PARP-1–dependent cell death.
  

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© 2011 American Association for the Advancement of Science