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Sci. Signal., 19 April 2011
[DOI: 10.1126/scisignal.2001309]

Supplementary Materials for:

Inhibitory ITAM Signaling Traps Activating Receptors with the Phosphatase SHP-1 to Form Polarized "Inhibisome" Clusters

Séverine Pfirsch-Maisonnas, Meryem Aloulou, Ting Xu, Julien Claver, Yutaka Kanamaru, Meetu Tiwari, Pierre Launay, Renato C. Monteiro,* Ulrich Blank*

*To whom correspondence should be addressed. E-mail: ulrich.blank{at}inserm.fr (U.B.); renato.monteiro{at}inserm.fr (R.C.M.)

This PDF file includes:

  • Fig. S1. Monovalent targeting of FcαRI by Fab A77, but not Fab A3, induces intracellular coclustering.
  • Fig. S2. Kinetics of Fab A77–induced inhibisome formation.
  • Fig. S3. Dose-response analysis of ITIM-dependent inhibition of β-hexosaminidase release.
  • Fig. S4. FcεRI localizes to distinct intracellular compartments after treatment with Fab A77 or Fab 320.
  • Fig. S5. SHP-1 and FcαRI colocalize in inhibisomes after targeting with the natural ligand IgA and in cells expressing endogenous FcαRI.
  • Fig. S6. Analysis of the efficacy of SHP-1 knockdown.
  • Fig. S7. Analysis of additional SHP-1–specific shRNAs for their capacity to reverse Fab A77–induced inhibition of β-hexosaminidase release.
  • Fig. S8. Effects of cytochalasin D and latrunculin B on F-actin content and SHP-1 localization in inhibisomes.
  • Legend for Movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

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Citation: S. Pfirsch-Maisonnas, M. Aloulou, T. Xu, J. Claver, Y. Kanamaru, M. Tiwari, P. Launay, R. C. Monteiro, U. Blank, Inhibitory ITAM Signaling Traps Activating Receptors with the Phosphatase SHP-1 to Form Polarized "Inhibisome" Clusters. Sci. Signal. 4, ra24 (2011).

© 2011 American Association for the Advancement of Science


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