Male Fertility Depends on Ca²⁺ Absorption by TRPV6 in Epididymal Epithelia

Petra Weissgerber, Ulrich Kriebs, Volodymyr Tsvilovskyy, Jenny Olausson, Oliver Kretz, Christof Stoerger, Rudi Vennekens, Ulrich Wissenbach, Ralf Middendorff, Veit Flockerzi, Marc Freichel*

*To whom correspondence should be addressed. E-mail: marc.freichel{at}uks.eu

This PDF file includes:

• Materials and Methods
• Fig. S1. Normal segregation of the Trpv6^mt allele and weight gain in Trpv6^mt/mt mice.
• Fig. S2. Characterization of TRPV6 antibodies.
• Fig. S3. Conversion of the Trpv6^L2F2 allele into a Trpv6 null allele (Trpv6^–) by Cre-mediated recombination in mice.
• Fig. S4. Ca²⁺ concentration and cAMP content in wild-type and Trpv6^mt/mt sperm.
• Fig. S5. Alizarin red staining in the cauda epididymis and histological analysis of prostates from wild-type and Trpv6^mt/mt mice.
• References
  

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.avi and .mov format). Capacitated sperm from the cauda epididymis of wild-type mice.
• Movie S2 (.avi and .mov format). Capacitated sperm from the cauda epididymis of Trpv6^mt/mt mice.

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© 2011 American Association for the Advancement of Science