α-Catenin Is a Tumor Suppressor That Controls Cell Accumulation by Regulating the Localization and Activity of the Transcriptional Coactivator Yap1

Mark R. Silvis, Bridget T. Kreger, Wen-Hui Lien, Olga Klezovitch, G. Marianna Rudakova, Fernando D. Camargo, Dan M. Lantz, John T. Seykora, Valeri Vasioukhin*

*To whom correspondence should be addressed. E-mail: vvasiouk{at}fhcrc.org

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• Fig. S1. Generation of GFAP-Cre/αE-catenin^fl/fl mice.
• Fig. S2. Skin histology of GFAP-Cre/αE-catenin^fl/fl mice.
• Fig. S3. Increased rates of cell proliferation in αE-catenin^−/− stem and early progenitor cells.
• Fig. S4. Inflammatory response in hair follicle cysts and tumors from mice with conditional knockout of αE-catenin or of αE-catenin and p53.
• Fig. S5. Tumors in GFAP-Cre/αE-catenin^fl/fl mice are derived from αE-catenin^−/− keratinocytes.
• Fig. S6. Apoptosis in hair follicle cysts from mice with conditional knockout of αE-catenin or of αE-catenin and p53.
• Fig. S7. αE-catenin is necessary for contact-mediated inhibition of cell accumulation.
• Fig. S8. Ninety-six–well plate assay for contact inhibition of cell accumulation.
• Fig. S9. Efficient knockdown of gene targets after siRNA transfection in 96-well plate contact inhibition assay.
• Fig. S10. siRNA screen for genes required for αE-catenin–mediated contact inhibition.
• Fig. S11. Efficient knockdown of Yap1 after transfection of keratinocytes with siRNA oligos.
• Fig. S12. Nuclear localization of Yap1 in subconfluent αE-catenin^fl/fl and αE-catenin^−/− keratinocytes.
• Fig. S13. Increased nuclear localization of Yap1 in confluent αE-catenin^−/− keratinocytes.
• Fig. S14. Rescue of cytoplasmic localization of Yap1 in αE-catenin^−/− cells transduced with retroviruses expressing full-length αE-catenin.
• Fig. S15. Partial colocalization between αE-catenin and Yap1 in confluent keratinocytes.
• Fig. S16. Yap1 is nuclear in both dividing and nondividing subconfluent keratinocytes.
• Fig. S17. Western blot analysis of Hippo pathway proteins in cultured keratinocytes.
• Fig. S18. Quantitation of Yap1-specific phosphorylation on Ser¹²⁷.
• Fig. S19. Decreased abundance of Yap1 mRNA in αE-catenin^−/− keratinocytes.
• Fig. S20. Acute knockdown of αE-catenin reduces total Yap1 protein abundance.
• Fig. S21. Nuclear localization of Yap1 in αE-catenin^−/− cortical neural progenitor cells.
• Fig. S22. Hippo pathway siRNA screen on cultured αE-catenin^fl/fl (Ctrl) and αE-catenin^−/− (α-cat^−/−) keratinocytes.
• Fig. S23. Wild-type, but not S94A mutant, human Yap2 protein is necessary for increased cell proliferation in αE-catenin^−/− keratinocytes.
• Fig. S24. qRT-PCR analysis of Cyr61 expression in 4-month-old skin and tumors from αE-catenin^fl/fl (Ctrl) and GFAP-Cre/αE-catenin^fl/fl (α-cat cKO) mice.
• References

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© 2011 American Association for the Advancement of Science