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Sci. Signal., 31 May 2011
[DOI: 10.1126/scisignal.2001325]

Supplementary Materials for:

Integration of Activating and Inhibitory Receptor Signaling by Regulated Phosphorylation of Vav1 in Immune Cells

Sven Mesecke, Doris Urlaub, Hauke Busch, Roland Eils,* Carsten Watzl*

*To whom correspondence should be addressed. E-mail: r.eils{at}dkfz.de (R.E.); watzl{at}uni-hd.de (C.W.)

This PDF file includes:

  • Methods
  • Fig. S1. Quantification of cytosolic proteins by Western blotting analysis.
  • Fig. S2. Initial simulation results.
  • Fig. S3. Interclass parameter ratios.
  • Fig. S4. Results of the parameter fit for all 72 models.
  • Fig. S5. Quantification of the total amounts of pVav1 and pSFK after stimulation of NK cells.
  • Fig. S6. Comparison between predicted and experimentally observed pVav1 responses after treatment with PP1.
  • Table S1. Kinetic parameters used for modeling.
  • Table S2. Protein numbers and concentrations for different NK cell types.
  • Table S3. Table of shape functions and their analytical equations used for the classification of shapes generated in the parameter scan.
  • Table S4. Literature-derived and fitted kinetic rates.
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 985 KB

Other Supplementary Material for this manuscript includes the following:

  • MATLAB and SBML model files (.m and .xml format).

[Download MATLAB and SBML model files (Compressed)]

Technical Details

Format: MATLAB and XML. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 15.7 KB (compressed); 384 KB (decompressed)


Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
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Citation: S. Mesecke, D. Urlaub, H. Busch, R. Eils, C. Watzl, Integration of Activating and Inhibitory Receptor Signaling by Regulated Phosphorylation of Vav1 in Immune Cells. Sci. Signal. 4, ra36 (2011).

© 2011 American Association for the Advancement of Science


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