Antigen Potency and Maximal Efficacy Reveal a Mechanism of Efficient T Cell Activation

Omer Dushek,* Milos Aleksic, Richard J. Wheeler, Hao Zhang, Shaun-Paul Cordoba, Yan-Chun Peng, Ji-Li Chen, Vincenzo Cerundolo, Tao Dong, Daniel Coombs, Philip Anton van der Merwe*

*To whom correspondence should be addressed. E-mail: omer.dushek{at}path.ox.ac.uk (O.D.); anton.vandermerwe{at}path.ox.ac.uk (P.A.v.d.M.)

This PDF file includes:

• Methods
• Fig. S1. Comparison of the affinity model with three formulations of the productive hit rate model.
• Fig. S2. Productive hit rate model with internalization.
• Fig. S3. Structure of the gag peptide in complex with HLA-A2.
• Fig. S4. Complete dose-response data for the G10 TCR system.
• Fig. S5. Additional correlations between reaction parameters and functional responses for G10 CTLs.
• Fig. S6. Time-course data for additional pMHCs.
• Fig. S7. Confirmation of the time dependency of E_max and the time independency of EC₅₀.
• Fig. S8. Confirmation of the relationships between K_D and EC₅₀ and between k_off and E_max.
• Fig. S9. Simulated dose-response curves using protocols 1 and 2.
• Fig. S10. Histogram of estimated EC₅₀ and E_max values from protocols 1 and 2.
• Fig. S11. Standard deviations in the EC₅₀ histograms from protocols 1 and 2.
• Fig. S12. Effects of TCR-pMHC rebinding in a confinement time model on maximal antigen efficacy.
• Table S1. Reaction and functional parameters for the G10 TCR.
• Table S2. Reaction and functional parameters for the 1G4 TCR.
• References

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© 2011 American Association for the Advancement of Science