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Sci. Signal., 7 June 2011
[DOI: 10.1126/scisignal.2001430]

Supplementary Materials for:

Antigen Potency and Maximal Efficacy Reveal a Mechanism of Efficient T Cell Activation

Omer Dushek,* Milos Aleksic, Richard J. Wheeler, Hao Zhang, Shaun-Paul Cordoba, Yan-Chun Peng, Ji-Li Chen, Vincenzo Cerundolo, Tao Dong, Daniel Coombs, Philip Anton van der Merwe*

*To whom correspondence should be addressed. E-mail: omer.dushek{at}path.ox.ac.uk (O.D.); anton.vandermerwe{at}path.ox.ac.uk (P.A.v.d.M.)

This PDF file includes:

  • Methods
  • Fig. S1. Comparison of the affinity model with three formulations of the productive hit rate model.
  • Fig. S2. Productive hit rate model with internalization.
  • Fig. S3. Structure of the gag peptide in complex with HLA-A2.
  • Fig. S4. Complete dose-response data for the G10 TCR system.
  • Fig. S5. Additional correlations between reaction parameters and functional responses for G10 CTLs.
  • Fig. S6. Time-course data for additional pMHCs.
  • Fig. S7. Confirmation of the time dependency of Emax and the time independency of EC50.
  • Fig. S8. Confirmation of the relationships between KD and EC50 and between koff and Emax.
  • Fig. S9. Simulated dose-response curves using protocols 1 and 2.
  • Fig. S10. Histogram of estimated EC50 and Emax values from protocols 1 and 2.
  • Fig. S11. Standard deviations in the EC50 histograms from protocols 1 and 2.
  • Fig. S12. Effects of TCR-pMHC rebinding in a confinement time model on maximal antigen efficacy.
  • Table S1. Reaction and functional parameters for the G10 TCR.
  • Table S2. Reaction and functional parameters for the 1G4 TCR.
  • References

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Technical Details

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Citation: O. Dushek, M. Aleksic, R. J. Wheeler, H. Zhang, S.-P. Cordoba, Y.-C. Peng, J.-L. Chen, V. Cerundolo, T. Dong, D. Coombs, P. A. van der Merwe, Antigen Potency and Maximal Efficacy Reveal a Mechanism of Efficient T Cell Activation. Sci. Signal. 4, ra39 (2011).

© 2011 American Association for the Advancement of Science


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