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Sci. Signal., 14 June 2011
[DOI: 10.1126/scisignal.2001750]

Supplementary Materials for:

Structure of a Pentavalent G-Actin•MRTF-A Complex Reveals How G-Actin Controls Nucleocytoplasmic Shuttling of a Transcriptional Coactivator

Stéphane Mouilleron, Carola A. Langer, Sebastian Guettler, Neil Q. McDonald,* Richard Treisman*

*To whom correspondence should be addressed. E-mail: Neil.McDonald{at}cancer.org.uk (N.Q.M.); Richard.Treisman{at}cancer.org.uk (R.T.)

This PDF file includes:

  • Fig. S1. CD spectrum for MRTF-A RPEL domain.
  • Fig. S2. Arrangement of actin molecules and structural subdomains in the pentavalent actin•RPEL assembly compared with those in the actin filament.
  • Fig. S3. Individual RPEL motifs and their extensions engage one or two actins within the pentameric complex.
  • Fig. S4. Comparison of pentavalent and trivalent actin•RPEL domain complexes.
  • Fig. S5. Actin S2 exhibits higher temperature factors than other actins in the pentavalent actin•RPEL domain assembly.
  • Fig. S6. TLS refinement shows higher mobility of actin S2 in the crystal lattice.
  • Fig. S7. Comparison of the interactions of RPELs and spacers close to the C terminus of actin in the pentameric actin•RPEL domain assembly.
  • Fig. S8. Size exclusion chromatography of G-actin•RPEL peptide and actin•RPEL domain complexes.
  • Fig. S9. Nuclear import properties of spacer mutants.
  • Fig. S10. Relationships of the actin•RPEL assemblies to import and export.
  • References
  • Legends for movies S1 and S2

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.13 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1. The pentavalent actin•RPEL domain assembly.
  • Movie S2. RPEL-actins R1, R2, and R3 align around the crank axis, in intimate contact with the RPEL domain.

[Download Movies S1 and S2 (Compressed)]

Technical Details

Format: Quicktime Movie. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 5.37 MB (compressed); 5.43 MB (decompressed)


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  1. Create a temporary folder on your machine's hard drive.
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Citation: S. Mouilleron, C. A. Langer, S. Guettler, N. Q. McDonald, R. Treisman, Structure of a Pentavalent G-Actin•MRTF-A Complex Reveals How G-Actin Controls Nucleocytoplasmic Shuttling of a Transcriptional Coactivator. Sci. Signal. 4, ra40 (2011).

© 2011 American Association for the Advancement of Science


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