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Sci. Signal., 14 June 2011
[DOI: 10.1126/scisignal.2001538]

Supplementary Materials for:

TRPS1 Targeting by miR-221/222 Promotes the Epithelial-to-Mesenchymal Transition in Breast Cancer

Susanna Stinson, Mark R. Lackner, Alex T. Adai, Nancy Yu, Hyo-Jin Kim, Carol O'Brien, Jill Spoerke, Suchit Jhunjhunwala, Zachary Boyd, Thomas Januario, Robert J. Newman, Peng Yue, Richard Bourgon, Zora Modrusan, Howard M. Stern, Søren Warming, Frederic J. de Sauvage, Lukas Amler, Ru-Fang Yeh, David Dornan*

*To whom correspondence should be addressed. E-mail: dornan.david{at}gene.com

This PDF file includes:

  • Materials and Methods
  • References
  • Fig. S1. Plot of miR-221, miR-222, and miR-200c expression across luminal and basal-like cell lines.
  • Fig. S2. Triple-negative breast cancers have more abundant miR-221/222 than ER/PR breast cancers.
  • Fig. S3. Phase contrast and immunofluorescence images of MCF10A cells transfected with miR-221/222.
  • Fig. S4. miR-221/222 abundance correlates with that of the mRNA encoding vimentin.
  • Fig. S5. miR-221/222 abundance inversely correlates with that of the mRNA encoding E-cadherin.
  • Fig. S6. miR-221/222 abundance inversely correlates with that of the mRNA encoding TRPS1.
  • Table S1. Luminal-basal specific differential gene expression.
  • Table S2. miR-221/222 gene targets down-regulated in basal-like subtype and in MCF10A cells overexpressing miR-221/222.
  • Table S3. Filtered candidate target genes for miR-221/222 based on CRS.

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.71 MB

 


Citation: S. Stinson, M. R. Lackner, A. T. Adai, N. Yu, H.-J. Kim, C. O'Brien, J. Spoerke, S. Jhunjhunwala, Z. Boyd, T. Januario, R. J. Newman, P. Yue, R. Bourgon, Z. Modrusan, H. M. Stern, S. Warming, F. J. de Sauvage, L. Amler, R.-F. Yeh, D. Dornan, TRPS1 Targeting by miR-221/222 Promotes the Epithelial-to-Mesenchymal Transition in Breast Cancer. Sci. Signal. 4, ra41 (2011).

© 2011 American Association for the Advancement of Science


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