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Sci. Signal., 26 July 2011
[DOI: 10.1126/scisignal.2001902]

Supplementary Materials for:

Proteome-Wide Mapping of the Drosophila Acetylome Demonstrates a High Degree of Conservation of Lysine Acetylation

Brian T. Weinert, Sebastian A. Wagner, Heiko Horn, Peter Henriksen, Wenshe R. Liu, Jesper V. Olsen, Lars J. Jensen, Chunaram Choudhary*

*To whom correspondence should be addressed. E-mail: chuna.choudhary{at}cpr.ku.dk

This PDF file includes:

  • Fig. S1. Functional annotation of Drosophila and human acetylomes.
  • Fig. S2. Conservation of serine and threonine phosphorylation sites to either serine or threonine.
  • Fig. S3. Summary of acetylation sites identified in E2-conjugating enzymes.
  • Fig. S4. Sequence alignment of human E2 ubiquitin-conjugating enzymes.
  • Fig. S5. Identification of UBC4 Lys9 acetylation in S. cerevisiae.
  • Fig. S6. Identification of in vivo UBE2D3 Lys8 acetylation in human cells.
  • Fig. S7. Confirmation of Lys8 acetylation in recombinant UBE2D3 purified from E. coli.
  • Fig. S8. Effect of UBE2D3 Lys8 acetylation on ubiquitin thiolester formation.
  • Fig. S9. Rescue of growth sensitivity in S. cerevisiae ubc4 mutant cells.
  • Definitions of the columns for Tables S1 to S3
  • Details regarding data availability

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 913 KB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of Drosophila in vivo acetylation sites.
  • Table S2 (Microsoft Excel format). Drosophila acetylated lysine conservation.
  • Table S3 (Microsoft Excel format). Human acetylated lysine conservation.

[Download Tables S1 to S3 (Compressed)]

Technical Details

Format: Microsoft Excel. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 1 MB (compressed); 3.17 MB (decompressed)


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Citation: B. T. Weinert, S. A. Wagner, H. Horn, P. Henriksen, W. R. Liu, J. V. Olsen, L. J. Jensen, C. Choudhary, Proteome-Wide Mapping of the Drosophila Acetylome Demonstrates a High Degree of Conservation of Lysine Acetylation. Sci. Signal. 4, ra48 (2011).

© 2011 American Association for the Advancement of Science


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