Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. Signal., 13 September 2011
[DOI: 10.1126/scisignal.2001936]

Supplementary Materials for:

Protein Arginine Methyltransferase 5 Regulates ERK1/2 Signal Transduction Amplitude and Cell Fate Through CRAF

Pedro Andreu-Pérez, Rosaura Esteve-Puig, Carlos de Torre-Minguela, Marta López-Fauqued, Joan Josep Bech-Serra, Stephan Tenbaum, Elena R. García-Trevijano, Francesc Canals, Glenn Merlino, Matías A. Ávila, Juan A. Recio*

*To whom correspondence should be addressed. E-mail: juan.recio{at}

This PDF file includes:

  • Fig. S1. MTA increases ERK1/2 phosphorylation in response to HGF in primary rat hepatocytes.
  • Fig. S2. Modulation of HGF induced MEK1/2-ERK1/2 signaling by MTA in melanoma cells.
  • Fig. S3. MTA modulates ERK1/2 phosphorylation upon specific growth factor treatment in human cells, and protein methylation inhibition modulates ERK1/2 signal amplitude in response to HGF.
  • Fig. S4. PRMT1, PRMT3, and PRMT8 are not responsible for the MTA-mediated effect.
  • Fig. S5. MTA signal amplitude modulation of ERK1/2 is RAS activation–dependent.
  • Fig. S6. CRAF and BRAF immunoprecipitate and colocalize with PRMT5 in the cytoplasm.
  • Fig. S7. MTA regulates growth factor–induced BRAF kinase activity.
  • Fig. S8. Differential activation of RAF isoforms by growth factors.
  • Fig. S9. MTA treatment inhibits CRAF degradation in SKMel147 cells.
  • Fig. S10. BRAFR671K mutant shows the same response as CRAFR563K. ARAF stability is not regulated by MTA in SKMel147 cells.
  • Fig. S11. A small fraction of total CRAF is recruited to the membrane in response to growth factor signaling. Detection by MALDI of both the unmethylated and the methylated CRAF peptides.
  • Fig. S12. BRAF is in vivo methylated. BRAFR671K mutant mimics the MTA-induced effects on PC12 cells.
  • Fig. S13. PC12 differentiation assay.
  • Methods
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 10.5 MB

Citation: P. Andreu-Pérez, R. Esteve-Puig, C. de Torre-Minguela, M. López-Fauqued, J. J. Bech-Serra, S. Tenbaum, E. R. García-Trevijano, F. Canals, G. Merlino, M. A. Ávila, J. A. Recio, Protein Arginine Methyltransferase 5 Regulates ERK1/2 Signal Transduction Amplitude and Cell Fate Through CRAF. Sci. Signal. 4, ra58 (2011).

© 2011 American Association for the Advancement of Science

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882