Supplementary Materials for:
Shigella flexneri Infection Generates the Lipid PI5P to Alter Endocytosis and Prevent Termination of EGFR Signaling
Damien Ramel, Frédéric Lagarrigue, Véronique Pons, Joëlle Mounier,
Sophie Dupuis-Coronas, Gaëtan Chicanne, Philippe J. Sansonetti,
Frédérique Gaits-Iacovoni, Hélène Tronchère, Bernard Payrastre*
*To whom correspondence should be addressed. E-mail: bernard.payrastre{at}inserm.fr
This PDF file includes:
- Fig. S1. Exogenous short-chain PI5P induces activation of EGFR and protects EGFR
from degradation.
- Fig. S2. Quantification of immunofluorescence and Western blots corresponding to
Fig. 1.
- Fig. S3. Phosphoinositide concentrations in HeLa cells expressing EGFP, EGFP-IpgD-WT, or EGFP-Inp54p.
- Fig. S4. Characterization of the Tet-Off IpgD-WT HeLa stable cell line.
- Fig. S5. PI5P production activates EGFR in the absence of detectable ligand.
- Fig. S6. Quantification of Western blots corresponding to Fig. 2F.
- Fig. S7. Cholesterol depletion attenuates IpgD-induced Akt activation.
- Fig. S8. Quantification of Western blots corresponding to Fig. 3A.
- Fig. S9. Transformation of PI(4,5)P2 into PI5P, but not into PI4P, changes EGFR
localization.
- Fig. S10. PI5P production localizes EGFR to SNX1-positive early endosomes and
prevents its routing to lysosomes.
- Fig. S11. PI5P production does not affect retrograde transport.
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Citation: D. Ramel, F. Lagarrigue, V. Pons, J. Mounier, S. Dupuis-Coronas, G. Chicanne,
P. J. Sansonetti, F. Gaits-Iacovoni, H. Tronchère, B. Payrastre,
Shigella flexneri Infection Generates the Lipid PI5P to Alter
Endocytosis and Prevent Termination of EGFR Signaling.
Sci. Signal. 4, ra61 (2011).
© 2011 American Association for the Advancement of Science
